ArticleJournal of clinical microbiology2004
Stable and noncompetitive RNA internal control for routine clinical diagnostic reverse transcription-PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 45 citations in OpenAlex.
- ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay.Scientific reports · 2024Article
- Wastewater-based surveillance as a tool for public health action: SARS-CoV-2 and beyond.Clinical microbiology reviews · 2024Review
- Simple Low-Cost Production of DNA MS2 Virus-Like Particles As Molecular Diagnostic Controls.GEN biotechnology · 2022Article
- Human Coronavirus NL63 Among Other Respiratory Viruses in Clinical Specimens of Egyptian Children and Raw Sewage Samples.Food and environmental virology · 2021Article
- The origin of exosomal miR-1246 in human cancer cells.RNA biology · 2019Article
- Internal control for real-time polymerase chain reaction based on MS2 bacteriophage for RNA viruses diagnostics.Memorias do Instituto Oswaldo Cruz · 2017Article
- Methods for Preparation of MS2 Phage-Like Particles and Their Utilization as Process Control Viruses in RT-PCR and qRT-PCR Detection of RNA Viruses From Food Matrices and Clinical Specimens.Food and environmental virology · 2015Article
- Viral Co-Infections in Pediatric Patients Hospitalized with Lower Tract Acute Respiratory Infections.PloS one · 2015Article
- Universal single-probe RT-PCR assay for diagnosis of dengue virus infections.PLoS neglected tropical diseases · 2014Article
- Optimization of PCR for quantification of simian immunodeficiency virus genomic RNA in plasma of rhesus macaques (Macaca mulatta) using armored RNA.Journal of medical primatology · 2014Article
- Hepatitis delta virus infects the cells of hepadnavirus-induced hepatocellular carcinoma in woodchucks.Hepatology (Baltimore, Md.) · 2012Article
- Failure to detect Xenotropic murine leukaemia virus-related virus in Chinese patients with chronic fatigue syndrome.Virology journal · 2010Article
- Oseltamivir-resistant pandemic (H1N1) 2009 in patient with impaired immune system.Emerging infectious diseases · 2010Article
- A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control.Virology journal · 2010Article
- Development of duplex real-time PCR for detection of two DNA respiratory viruses.Journal of virological methods · 2009Article
- Internal control for nucleic acid testing based on the use of purified Bacillus atrophaeus subsp. globigii spores.Journal of clinical microbiology · 2009Article
- Currently used nucleic acid amplification tests for the detection of viruses and atypicals in acute respiratory infections.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Strategic approach to produce low-cost, efficient, and stable competitive internal controls for detection of RNA viruses by use of reverse transcription-PCR.Journal of clinical microbiology · 2007Article
- Nucleic acid amplification tests for detection of respiratory viruses.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2007Review
- Multiplexed reverse transcriptase PCR assay for identification of viral respiratory pathogens at the point of care.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clinical diagnostic tests based on nucleic acid amplification assist with the prompt diagnosis of microbial infections because of their speeds and extremely low limits of detection. However, the design of appropriate internal controls for such assays has proven difficult. We describe a reaction-specific RNA internal control for diagnostic reverse transcription (RT)-PCR which allows extraction, RT, amplification, and detection to be monitored. The control consists of a G+C-rich (60%) RNA molecule with an extensive secondary structure, based on a modified hepatitis delta virus genome. The rod-like structure of this RNA, with 70% intramolecular base pairing, provides a difficult template for RT-PCR. This ensures that the more favorable target virus amplicon is generated in preference to the control, with the control being detected only if the target virus is absent. The unusual structure of hepatitis delta virus RNA has previously been shown to enhance its stability and resistance to nucleases, an advantage for routine use as an internal control. The control was implemented in three nested multiplex RT-PCRs to detect nine clinically important respiratory viruses: (i) influenza A and B viruses, (ii) respiratory syncytial viruses A and B and human metapneumovirus, and (iii) parainfluenza virus types 1 to 4. The detection limits of these assays were not detectably compromised by the presence of the RNA control. During routine testing of 324 consecutive unselected respiratory samples, the presence of the internal control ensured that genuine and false-negative results were distinguishable, thus increasing the diagnostic confidence in the assay.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.