ArticleThe Journal of experimental medicine2004
Mapping of a functional recombination motif that defines isotype specificity for mu-->gamma3 switch recombination implicates NF-kappaB p50 as the isotype-specific switching factor.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Differential regulation of immunoglobulin class switch recombination by the tumor suppressor p53.Scientific reports · 2026Article
- LRRK1 is critical in the regulation of B-cell responses and CARMA1-dependent NF-κB activation.Scientific reports · 2016Article
- Three-dimensional architecture of the IgH locus facilitates class switch recombination.Annals of the New York Academy of Sciences · 2012Article
- Impaired B cell development and function in the absence of IkappaBNS.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Expression of IgA class switching gene in tonsillar mononuclear cells in patients with IgA nephropathy.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2011Article
- Switch region identity plays an important role in Ig class switch recombination.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Roles of the NF-kappaB pathway in lymphocyte development and function.Cold Spring Harbor perspectives in biology · 2010Review
- Binding of LBP-1a to specific immunoglobulin switch regions in vivo correlates with specific repression of class switch recombination.European journal of immunology · 2009Article
- Sgamma3 switch sequences function in place of endogenous Sgamma1 to mediate antibody class switching.The Journal of experimental medicine · 2008Article
- Mechanism and regulation of class switch recombination.Annual review of immunology · 2008Review
- Article
- Viral induction of AID is independent of the interferon and the Toll-like receptor signaling pathways but requires NF-kappaB.The Journal of experimental medicine · 2007Article
- NF-kappa B binds to the immunoglobulin S gamma 3 region in vivo during class switch recombination.European journal of immunology · 2006Article
- AID-dependent histone acetylation is detected in immunoglobulin S regions.The Journal of experimental medicine · 2006Article
- Influence of switch region length on immunoglobulin class switch recombination.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- AID: a very old motif newly recognized.Nature immunology · 2004Article
- Differential regulation of histone acetylation and generation of mutations in switch regions is associated with Ig class switching.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Ig class switch recombination (CSR) requires expression of activation-induced deaminase (AID) and production of germline transcripts to target S regions for recombination. However, the mechanism of CSR remains unclear. Here we show that an extrachromosomal S plasmid assay is AID dependent and that a single consensus repeat is both necessary and sufficient for isotype-specific CSR. Transfected switch substrates specific for mu-->gamma3 and mu-->gamma1 are stimulated to switch with lipopolysaccharide (LPS) alone or LPS and interleukin-4, respectively. An Sgamma3/Sgamma1 substrate containing only three Sgamma3-associated nucleotides reconstituted LPS responsiveness and permitted mapping of a functional recombination motif specific for mu-->gamma3 CSR. This functional recombination motif colocalized with a binding site for NF-kappaB p50, and p50 binding to this site was previously established. We show a p50 requirement for plasmid-based mu-->gamma3 CSR using p50-deficient B cells. Switch junctions from p50-deficient B cells showed decreased lengths of microhomology between Smu and Sgamma3 relative to wild-type cells, indicating a function for p50 in the mechanics of CSR. We note a striking parallel between the affects of p50 and Msh2 deficiency on Smu/Sgamma3 junctions. The data suggest that p50 may be the isotype-specific factor in mu-->gamma3 CSR and epistatic with Msh2.
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