ArticleGenome research2004
Novel RNAs identified from an in-depth analysis of the transcriptome of human chromosomes 21 and 22.
Article in Genome research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 215 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
215 citing papers in PubMed.
- Trial
- Long Non-Coding RNAs: Key Regulators of Economic Traits in Livestock From Mechanisms to Breeding Applications.Veterinary medicine and science · 2026Review
- Short-read RNA-seq yields lower estimates of A-to-I RNA editing levels than long-read cDNA sequencing.Advanced biotechnology · 2026Article
- The splice of life: an isoform-centric view of disease, technology, and therapeutics.The Journal of clinical investigation · 2026Review
- Influence of MgACS omega · 2023Article
- HOX-Gene Cluster Organization and Genome Duplications in Fishes and Mammals: Transcript Variant Distribution along the Anterior-Posterior Axis.International journal of molecular sciences · 2022Review
- Cataloguing the proteome: Current developments in single-molecule protein sequencing.Biophysics reviews · 2022Review
- Review
- Long Non-coding RNA Expression Profiling Using Arraystar LncRNA Microarrays.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Developmental Expression of SULT1C4 Transcript Variants in Human Liver: Implications for Discordance Between SULT1C4 mRNA and Protein Levels.Drug metabolism and disposition: the biological fate of chemicals · 2020Article
- Bringing MicroRNAs to Light: Methods for MicroRNA Quantification and Visualization in Live Cells.Frontiers in bioengineering and biotechnology · 2020Review
- Do Human Endogenous Retroviruses Contribute to Multiple Sclerosis, and if So, How?Molecular neurobiology · 2019Review
- Potential Implications of Long Noncoding RNAs in Autoimmune Diseases.Immune network · 2019Review
- RNA-Seq Perspectives to Improve Clinical Diagnosis.Frontiers in genetics · 2019Review
- Expression analysis of lncRNA AK370814 involved in the barley vitamin B6 salvage pathway under salinity.Molecular biology reports · 2018Article
- iSeg: an efficient algorithm for segmentation of genomic and epigenomic data.BMC bioinformatics · 2018Article
- siRNAs regulate DNA methylation and interfere with gene and lncRNA expression in the heterozygous polyploid switchgrass.Biotechnology for biofuels · 2018Article
- Deciphering Non-coding RNAs in Cardiovascular Health and Disease.Frontiers in cardiovascular medicine · 2018Review
- Transcriptional and Post-transcriptional Gene Regulation by Long Non-coding RNA.Genomics, proteomics & bioinformatics · 2017Review
- Cryptochrome 2 extensively regulates transcription of the chloroplast genome in tomato.FEBS open bio · 2017Article
155 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors.
Funding
Abstract
In this report, we have achieved a richer view of the transcriptome for Chromosomes 21 and 22 by using high-density oligonucleotide arrays on cytosolic poly(A)(+) RNA. Conservatively, only 31.4% of the observed transcribed nucleotides correspond to well-annotated genes, whereas an additional 4.8% and 14.7% correspond to mRNAs and ESTs, respectively. Approximately 85% of the known exons were detected, and up to 21% of known genes have only a single isoform based on exon-skipping alternative expression. Overall, the expression of the well-characterized exons falls predominately into two categories, uniquely or ubiquitously expressed with an identifiable proportion of antisense transcripts. The remaining observed transcription (49.0%) was outside of any known annotation. These novel transcripts appear to be more cell-line-specific and have lower and less variation in expression than the well-characterized genes. Novel transcripts were further characterized based on their distance to annotations, transcript size, coding capacity, and identification as antisense to intronic sequences. By RT-PCR, 126 novel transcripts were independently verified, resulting in a 65% verification rate. These observations strongly support the argument for a re-evaluation of the total number of human genes and an alternative term for "gene" to encompass these growing, novel classes of RNA transcripts in the human genome.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.