ArticleNeurochemical research2004
Choline acetyltransferase: regulation and coupling with protein kinase and vesicular acetylcholine transporter on synaptic vesicles.
Article in Neurochemical research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Research Progress in the Effect of Traditional Chinese Medicine for Invigoration on Neurotransmitter Related Diseases.Evidence-based complementary and alternative medicine : eCAM · 2018Review
- Rethinking inflammation: neural circuits in the regulation of immunity.Immunological reviews · 2012Review
- Chronic cerebral ischaemia forms new cholinergic mechanisms of learning and memory.International journal of Alzheimer's disease · 2010Article
- Role of mu-calpain in proteolytic cleavage of brain L-glutamic acid decarboxylase.Brain research · 2008Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Both the membrane-bound choline acetyltransferase (MChAT) and soluble ChAT (SChAT) were found to be activated by ATP-mediated protein phosphorylation. ATP activation of MChAT but not SChAT was found to depend on the integrity of proton gradient of synaptic vesicles because conditions disrupting the proton gradient also abolished the activation of MChAT by ATP. Among the kinases studied, Ca2+/calmodulin kinase II is most effective in activation of MChAT. Transport of ACh into synaptic vesicles by vesicular acetylcholine transporter (VAChT) is also proton gradient-dependent; therefore we proposed that there is a functional coupling between ACh synthesis and its packaging into synaptic vesicles. This notion is supported by the following findings: first, the newly synthesized [3H]-ACh from [3H]-choline was taken up much more efficiently than the pre-existing ACh; second, ATP-activation of MChAT was abolished when VAChT was inhibited by the specific inhibitor vesamicol; third, the activity of ChAT was found to be markedly increased when neurons are under depolarizing conditions.
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Registered trials
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