ArticleJournal of virology2004
Assessment of the role of the central DNA flap in human immunodeficiency virus type 1 replication by using a single-cycle replication system.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Structure-specific nucleases in genome dynamics and strategies for targeting cancers.Journal of molecular cell biology · 2024Review
- Noncovalent SUMO-interaction motifs in HIV integrase play important roles in SUMOylation, cofactor binding, and virus replication.Virology journal · 2019Article
- Reverse Transcription of Retroviruses and LTR Retrotransposons.Microbiology spectrum · 2015Review
- Human immunodeficiency virus type 1 employs the cellular dynein light chain 1 protein for reverse transcription through interaction with its integrase protein.Journal of virology · 2015Article
- Viral and cellular requirements for the nuclear entry of retroviral preintegration nucleoprotein complexes.Viruses · 2013Review
- The importance of becoming double-stranded: Innate immunity and the kinetic model of HIV-1 central plus strand synthesis.Virology · 2013Review
- A protein ballet around the viral genome orchestrated by HIV-1 reverse transcriptase leads to an architectural switch: from nucleocapsid-condensed RNA to Vpr-bridged DNA.Virus research · 2013Article
- Proteomic analysis of early HIV-1 nucleoprotein complexes.Journal of proteome research · 2013Article
- Slower uncoating is associated with impaired replicative capability of simian-tropic HIV-1.PloS one · 2013Article
- Human immunodeficiency virus reverse transcriptase: 25 years of research, drug discovery, and promise.The Journal of biological chemistry · 2012Review
- The cellular antiviral protein APOBEC3G interacts with HIV-1 reverse transcriptase and inhibits its function during viral replication.Journal of virology · 2012Article
- Contribution of host nucleoporin 62 in HIV-1 integrase chromatin association and viral DNA integration.The Journal of biological chemistry · 2012Article
- Differential effects of Vpr on single-cycle and spreading HIV-1 infections in CD4+ T-cells and dendritic cells.PloS one · 2012Article
- The HIV-1 passage from cytoplasm to nucleus: the process involving a complex exchange between the components of HIV-1 and cellular machinery to access nucleus and successful integration.International journal of biochemistry and molecular biology · 2012Article
- Thriving under stress: selective translation of HIV-1 structural protein mRNA during Vpr-mediated impairment of eIF4E translation activity.PLoS pathogens · 2012Article
- Residual HIV-1 DNA Flap-independent nuclear import of cPPT/CTS double mutant viruses does not support spreading infection.Retrovirology · 2011Article
- Host protein Ku70 binds and protects HIV-1 integrase from proteasomal degradation and is required for HIV replication.The Journal of biological chemistry · 2011Article
- Revisiting HIV-1 uncoating.Retrovirology · 2010Review
- The HIV-1 central polypurine tract functions as a second line of defense against APOBEC3G/F.Journal of virology · 2010Article
- Importin alpha3 interacts with HIV-1 integrase and contributes to HIV-1 nuclear import and replication.Journal of virology · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, reverse transcriptase (RT)- and integrase (IN)-defective human immunodeficiency virus type 1 (HIV-1) was transcomplemented with Vpr-RT-IN fusion proteins to delineate pol sequences important for HIV-1 replication. Our results reveal that a 194-bp sequence encompassing the 3'end of the IN gene and containing the central DNA flap is necessary and sufficient for efficient HIV-1 single-cycle replication in dividing and nondividing cells. Furthermore, we show that the central DNA flap enhances HIV-1 single-round replication by five- to sevenfold, primarily by facilitating nuclear import of proviral DNA. In agreement with previous reports, our data support a functional role of the central DNA flap during the early stages of HIV-1 infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.