ArticleBiophysical journal2004
Nanometer analysis of cell spreading on matrix-coated surfaces reveals two distinct cell states and STEPs.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed.
- Differential modulation of cellular phenotype and drug sensitivity by extracellular matrix proteins in primary and metastatic pancreatic cancer cells.Molecular biology of the cell · 2023Article
- Crosslinking strategies for biomimetic hydrogels in bone tissue engineering.Biophysical reviews · 2023Review
- Integrating shear flow and trypsin treatment to assess cell adhesion strength.Biointerphases · 2023Article
- Mechanisms of frustrated phagocytic spreading of human neutrophils on antibody-coated surfaces.Biophysical journal · 2022Article
- Integrative experimental/computational approach establishes active cellular protrusion as the primary driving force of phagocytic spreading by immune cells.PLoS computational biology · 2022Article
- Modeling Receptor Motility along Advecting Lipid Membranes.Membranes · 2022Review
- Quantifying protrusions as tumor-specific biophysical predictors of cancer invasion in in vitro tumor micro-spheroid models.In vitro models · 2022Article
- Fabrication of physical and chemical crosslinked hydrogels for bone tissue engineering.Bioactive materials · 2022Review
- Adaptation of cell spreading to varying fibronectin densities and topographies is facilitated by β1 integrins.Frontiers in bioengineering and biotechnology · 2022Article
- High-Throughput Methods in the Discovery and Study of Biomaterials and Materiobiology.Chemical reviews · 2021Review
- Article
- Development of Nascent Focal Adhesions in Spreading Cells.Biophysical journal · 2020Review
- Human Salivary Histatin-1 Promotes Osteogenic Cell Spreading on Both Bio-Inert Substrates and Titanium SLA Surfaces.Frontiers in bioengineering and biotechnology · 2020Article
- Article
- Universal Kinetics of the Onset of Cell Spreading on Substrates of Different Stiffness.Biophysical journal · 2019Article
- Dynamic microtubules drive fibroblast spreading.Biology open · 2018Article
- Lamellipodium is a myosin-independent mechanosensor.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mapping Cell Membrane Fluctuations Reveals Their Active Regulation and Transient Heterogeneities.Biophysical journal · 2017Article
- Membrane tension controls adhesion positioning at the leading edge of cells.The Journal of cell biology · 2017Article
- Systems biology of cellular membranes: a convergence with biophysics.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
29 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
When mouse embryonic fibroblasts in suspension contact a matrix-coated surface, they rapidly adhere and spread. Using total internal reflection fluorescence microscopy of dye-loaded fibroblasts to quantify cell-substrate contact, we found that increasing the surface matrix density resulted in faster spreading initiation whereas lamellipodial dynamics during spreading were unaltered. After spreading initiation, most cells spread in an anisotropic manner through stochastic, transient extension periods (STEPs) with approximately 30 STEPs over 10 min to reach an area of 1300 micro m(2) +/- 300 micro m(2). A second mode of spreading, increased in serum-deprived cells, lacked STEPs and spread in a rapid, isotropic manner for 1-4 min. This isotropic mode was characterized by a high rate of area increase, 340 micro m(2)/min with 78% of the cell edge extending. Anisotropic cells spread slower via STEPs, 126 micro m(2)/min with 34% of the edge extending. During the initial 2-4 min of fast, isotropic spreading, centripetal flow of actin was low (0.8 micro m/min) whereas in anisotropic cells it was high from early times (4.7 micro m/min). After initial isotropic spreading, rearward actin movement increased and isotropic cells displayed STEPs similar to anisotropic cells. Thus, the two cell states display dramatically different spreading whereas long-term motility is based on STEPs.
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