ArticleBiophysical journal2004
Elasticity and adhesion force mapping reveals real-time clustering of growth factor receptors and associated changes in local cellular rheological properties.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
46 citing papers in PubMed, 146 citations in OpenAlex.
- Applying the Atomic Force Microscopy Technique in Medical Sciences-A Narrative Review.Biomedicines · 2024Review
- Evaluation of Surface Structure and Morphological Phenomena of Caucasian Virgin Hair with Atomic Force Microscopy.Medicina (Kaunas, Lithuania) · 2024Article
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- Spatial high resolution of actin filament organization by PeakForce atomic force microscopy.Cell proliferation · 2020Article
- Single-Molecule Force Measurement Guides the Design of Multivalent Ligands with Picomolar Affinity.Angewandte Chemie (International ed. in English) · 2019Article
- Quantifying the phosphorylation timescales of receptor-ligand complexes: a Markovian matrix-analytic approach.Open biology · 2018Article
- Real-time visualization of clustering and intracellular transport of gold nanoparticles by correlative imaging.Nature communications · 2017Article
- Biophysical differences between chronic myelogenous leukemic quiescent and proliferating stem/progenitor cells.Nanomedicine : nanotechnology, biology, and medicine · 2016Article
- Quantitatively Resolving Ligand-Receptor Bonds on Cell Surfaces Using Force-Induced Remnant Magnetization Spectroscopy.ACS central science · 2016Article
- Review
- Article
- Biocompatible Optically Transparent MEMS for Micromechanical Stimulation and Multimodal Imaging of Living Cells.Annals of biomedical engineering · 2015Article
- Investigating cell mechanics with atomic force microscopy.Journal of the Royal Society, Interface · 2015Article
- Single cell profiling of surface carbohydrates on Bacillus cereus.Journal of the Royal Society, Interface · 2015Article
- AFM single-cell force spectroscopy links altered nuclear and cytoskeletal mechanics to defective cell adhesion in cardiac myocytes with a nuclear lamin mutation.Nucleus (Austin, Tex.) · 2015Article
- Atomic force microscopy determination of Young׳s modulus of bovine extra-ocular tendon fiber bundles.Journal of biomechanics · 2014Article
- Heterogeneous elastic response of human lung microvascular endothelial cells to barrier modulating stimuli.Nanomedicine : nanotechnology, biology, and medicine · 2013Article
- Multiparametric imaging of biological systems by force-distance curve-based AFM.Nature methods · 2013Review
- High performance, LED powered, waveguide based total internal reflection microscopy.Scientific reports · 2013Article
- Toxicity effects of short term diesel exhaust particles exposure to human small airway epithelial cells (SAECs) and human lung carcinoma epithelial cells (A549).Toxicology letters · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell surface macromolecules such as receptors and ion channels serve as the interface link between the cytoplasm and the extracellular region. Their density, distribution, and clustering are key spatial features influencing effective and proper physical and biochemical cellular responses to many regulatory signals. In this study, the effect of plasma-membrane receptor clustering on local cell mechanics was obtained from maps of interaction forces between antibody-conjugated atomic force microscope tips and a specific receptor, a vascular endothelial growth factor (VEGF) receptor. The technique allows simultaneous measurement of the real-time motion of specific macromolecules and their effect on local rheological properties like elasticity. The clustering was stimulated by online additions of VEGF, or antibody against VEGF receptors. VEGF receptors are found to concentrate toward the cell boundaries and cluster rapidly after the online additions commence. Elasticity of regions under the clusters is found to change remarkably, with order-of-magnitude stiffness reductions and fluidity increases. The local stiffness reductions are nearly proportional to receptor density and, being concentrated near the cell edges, provide a mechanism for cell growth and angiogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.