ArticleBiophysical journal2004
Characterization of a range of fura dyes with two-photon excitation.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
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- The evolution of organellar calcium mapping technologies.Cell calcium · 2022Review
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- Error correction due to background subtraction in ratiometric calcium measurements with CCD camera.Heliyon · 2020Article
- Approaches to High-Throughput Analysis of Cardiomyocyte Contractility.Frontiers in physiology · 2020Article
- Systemic isradipine treatment diminishes calcium-dependent mitochondrial oxidant stress.The Journal of clinical investigation · 2018Article
- Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2017Article
- A technical review of optical mapping of intracellular calcium within myocardial tissue.American journal of physiology. Heart and circulatory physiology · 2016Review
- Arduino Due based tool to facilitate in vivo two-photon excitation microscopy.Biomedical optics express · 2016Article
- Automated analysis of contractile force and Ca2+ transients in engineered heart tissue.American journal of physiology. Heart and circulatory physiology · 2014Article
- Synthesis and properties of Asante Calcium Red--a novel family of long excitation wavelength calcium indicators.Cell calcium · 2013Article
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- Calcium signaling in dendritic spines.Cold Spring Harbor perspectives in biology · 2012Article
- Optical imaging of voltage and calcium in cardiac cells & tissues.Circulation research · 2012Review
- K201 (JTV-519) alters the spatiotemporal properties of diastolic Ca(2+) release and the associated diastolic contraction during β-adrenergic stimulation in rat ventricular cardiomyocytes.Basic research in cardiology · 2011Article
- Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Metal Ion-Responsive Fluorescent Probes for Two-Photon Excitation Microscopy.Chemistry of materials : a publication of the American Chemical Society · 2011Article
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3 authors at 2 institutions in 1 country.
Funding
Abstract
Two-photon excitation (TPE) spectra of Fura-2, -4F, -6F, -FF, and Furaptra were characterized using a tunable (750-850 nM) ultra-short pulse laser. Two-photon fluorescence of these dyes was studied in free solution and in the cytosol of isolated rabbit ventricular cardiomyocytes. The TPE spectra of the Ca(2+)-free and Ca(2+)-bound forms of the dyes were measured in free solution and expressed in terms of the two-photon fluorescence cross section (Goppert-Meyer units). The Fura dyes displayed the same Ca(2+)-free TPE spectrum in the intracellular volume of permeabilized and intact cardiomyocytes. Fluorescence measurements over a range of laser powers confirmed the TPE of both Ca(2+)-free and Ca(2+)-bound forms of the dyes. Single-wavelength excitation at 810 nM was used to determine the effective dissociation constants (K(eff)) and dynamic ranges (R(f)) of Fura-2, -4F, -6F, -FF, and Furaptra dyes (K(eff) = 181 +/- 52 nM, 1.16 +/- 0.016 micro M, 5.18 +/- 0.3 micro M, 19.2 +/- 1 micro M, and 58.5 +/- 2 micro M; and R(f) = 22.4 +/- 3.8, 12.2 +/- 0.34, 6.3 +/- 0.17, 16.1 +/- 2.8, and 25.4 +/- 4, respectively). Single-wavelength excitation of intracellular Fura-4F resolved diastolic and peak [Ca(2+)] in isolated stimulated cardiomyocytes after calibration of the intracellular signal using reversible exposure to low (100 micro M) extracellular [Ca(2+)]. Furthermore, TPE of Fura-4F allowed continuous, long-term (5-10 min) Ca(2+) imaging in ventricular cardiomyocytes using laser-scanning microscopy without significant cellular photodamage or photobleaching of the dye.
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