ArticleBiophysical journal2004
Protein thermal aggregation involves distinct regions: sequential events in the heat-induced unfolding and aggregation of hemoglobin.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Effect of Boiling onFoods (Basel, Switzerland) · 2025Article
- Refolding of Hemoglobin Under Macromolecular Confinement: Impersonating In Vivo Volume Exclusion.Journal of fluorescence · 2021Article
- Hemoglobin structure at higher levels of hemoglobin A1C in type 2 diabetes and associated complications.Chinese medical journal · 2020Article
- The Interplay between Molten Globules and Heme Disassociation Defines Human Hemoglobin Disassembly.Biophysical journal · 2020Article
- Kinetic stability of membrane proteins.Biophysical reviews · 2017Review
- Polyols (Glycerol and Ethylene glycol) mediated amorphous aggregate inhibition and secondary structure restoration of metalloproteinase-conalbumin (ovotransferrin).International journal of biological macromolecules · 2017Article
- The impact of the HbA1c level of type 2 diabetics on the structure of haemoglobin.Scientific reports · 2016Article
- Multi-Wavelength Photoacoustic Visualization of High Intensity Focused Ultrasound Lesions.Ultrasonic imaging · 2016Article
- Thermal-induced force release in oxyhemoglobin.Scientific reports · 2015Article
- Kinetics and thermodynamics of membrane protein folding.Biomolecules · 2014Review
- Thermal stability of high concentration lysozyme across varying pH: A Fourier Transform Infrared study.Journal of pharmacy & bioallied sciences · 2013Article
- Effects of spermine NONOate and ATP on protein aggregation: light scattering evidences.BMC biophysics · 2013Article
- Thermal, chemical and pH induced denaturation of a multimeric β-galactosidase reveals multiple unfolding pathways.PloS one · 2012Article
- Sequential events in the irreversible thermal denaturation of human brain-type creatine kinase by spectroscopic methods.International journal of molecular sciences · 2010Article
- Hemoglobin dynamics in red blood cells: correlation to body temperature.Biophysical journal · 2008Article
- Structural transition temperature of hemoglobins correlates with species' body temperature.European biophysics journal : EBJ · 2007Article
- Dissecting the pretransitional conformational changes in aminoacylase I thermal denaturation.Biophysical journal · 2007Article
- Body temperature-related structural transitions of monotremal and human hemoglobin.Biophysical journal · 2006Article
- Oligomerization and aggregation of bovine pancreatic ribonuclease A: characteristic events observed by FTIR spectroscopy.Biophysical journal · 2006Article
- Thermal aggregation of SARS-CoV membrane protein.Journal of virological methods · 2005Article
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4 authors.
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Abstract
Protein thermal aggregation plays a crucial role in protein science and engineering. Despite its biological importance, little is known about the mechanism and pathway(s) involved in the formation of aggregates. In this report, the sequential events occurring during thermal unfolding and aggregation process of hemoglobin were studied by two-dimensional infrared correlation spectroscopy. Analysis of the infrared spectra recorded at different temperatures suggested that hemoglobin denatured by a two-stage thermal transition. At the initial structural perturbation stage (30-44 degrees C), the fast red shift of the band from alpha-helix indicated that the native helical structures became more and more solvent-exposed as temperature increased. At the thermal unfolding stage (44-54 degrees C), the unfolding of solvent-exposed helical structures dominated the transition and was supposed to be responsible to the start of aggregation. At the thermal aggregation stage (54-70 degrees C), the transition was dominated by the formation of aggregates and the further unfolding of the buried structures. A close inspection of the sequential events occurring at different stages suggested that protein thermal aggregation involves distinct regions.
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