ArticleBiophysical journal2004
A multidimensional 1H NMR investigation of the conformation of methionine-enkephalin in fast-tumbling bicelles.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 109 citations in OpenAlex.
- Unveiling Dopamine and Met-Enkephalin Dynamics: Simultaneous Co-Detection in Rat Striatum.Analytical chemistry · 2025Article
- Involvement of the Opioid Peptide Family in Cancer Progression.Biomedicines · 2023Review
- Non-Canonical Amino Acids as Building Blocks for Peptidomimetics: Structure, Function, and Applications.Biomolecules · 2023Review
- Point-Substitution of Phenylalanine Residues of 26RFa Neuropeptide: A Structure-Activity Relationship Study.Molecules (Basel, Switzerland) · 2021Article
- Structure of Fungal α Mating Pheromone in Membrane Mimetics Suggests a Possible Role for Regulation at the Water-Membrane Interface.Frontiers in microbiology · 2020Article
- Tunable Coarse Graining for Monte Carlo Simulations of Proteins via Smoothed Energy Tables: Direct and Exchange Simulations.Journal of chemical theory and computation · 2014Article
- A new set of atomic radii for accurate estimation of solvation free energy by Poisson-Boltzmann solvent model.Journal of computational chemistry · 2014Article
- Topological analyses of time-dependent electronic structures: application to electron-transfers in methionine enkephalin.Journal of molecular modeling · 2014Article
- Lipid concentration and molar ratio boundaries for the use of isotropic bicelles.Langmuir : the ACS journal of surfaces and colloids · 2014Article
- When detergent meets bilayer: birth and coming of age of lipid bicelles.Progress in nuclear magnetic resonance spectroscopy · 2013Review
- The magic of bicelles lights up membrane protein structure.Chemical reviews · 2012Review
- Kinetics of binding and diffusivity of leucine-enkephalin in large unilamellar vesicle by pulsed-field-gradientBiophysics (Nagoya-shi, Japan) · 2011Article
- MOLS sampling and its applications in structural biophysics.Biophysical reviews · 2010Review
- Effect of sodium bicarbonate as a pharmaceutical formulation excipient on the interaction of fluvastatin with membrane phospholipids.European biophysics journal : EBJ · 2010Article
- Structural and antimicrobial properties of human pre-elafin/trappin-2 and derived peptides against Pseudomonas aeruginosa.BMC microbiology · 2010Article
- NCAD, a database integrating the intrinsic conformational preferences of non-coded amino acids.The journal of physical chemistry. B · 2010Article
- The C-F bond as a conformational tool in organic and biological chemistry.Beilstein journal of organic chemistry · 2010Article
- Membrane catalysis of peptide-receptor binding.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2010Review
- Conformational heterogeneity of a leucine enkephalin analogue in aqueous solution and sodium dodecyl sulfate micelles: comparison of time-resolved FRET and molecular dynamics simulations.The journal of physical chemistry. B · 2009Article
- Generalized pattern search algorithm for Peptide structure prediction.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enkephalins are pentapeptides found in the central nervous system. It is believed that these neuropeptides interact with the nerve cell membrane to adopt a conformation suitable for their binding to an opiate receptor. In this work, we have determined the three-dimensional structure of methionine-enkephalin (Menk) in fast-tumbling bicelles using multidimensional (1)H NMR. Bicelles were selected as model membranes because both their bilayer organization and composition resemble those of natural biomembranes. The effect of the membrane composition on the peptide conformation was explored using both zwitterionic (PC bicelles) and negatively charged bicelles (Bic/PG). Pulsed field gradient experiments allowed the determination of the proportion of Menk bound to the model membranes. Approximately 60% of the water-soluble enkephalin was found to associate to the bicellar systems. Structure calculations from torsion angle and NOE-based distance constraints suggest the presence of both micro - and delta-selective conformers of Menk in each system and slightly different conformers in PC bicelles and Bic/PG. As opposed to previous studies of enkephalins in membrane mimetic systems, our results show that these opiate peptides could adopt several conformations in a membrane environment, which is consistent with the flexibility and poor selectivity of enkephalins.
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