ArticleBiophysical journal2004
Lateral distribution of cholesterol in dioleoylphosphatidylcholine lipid bilayers: cholesterol-phospholipid interactions at high cholesterol limit.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 107 citations in OpenAlex.
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- Cholesterol and Lipid Rafts in the Biogenesis of Amyloid-β Protein and Alzheimer's Disease.Annual review of biophysics · 2024Review
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- Resveratrol and Resveratrol-Loaded Galactosylated Liposomes: Anti-Adherence and Cell Wall Damage Effects onBiomolecules · 2023Article
- Cholesterol in the cargo membrane amplifies tau inhibition of kinesin-1-based transport.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Interleaflet Decoupling in a Lipid Bilayer at Excess Cholesterol Probed by Spectroscopic Ellipsometry and Simulations.The Journal of membrane biology · 2020Article
- A new approach for investigating the response of lipid membranes to electrocompression by coupling droplet mechanics and membrane biophysics.Journal of the Royal Society, Interface · 2019Article
- Regimes of Complex Lipid Bilayer Phases Induced by Cholesterol Concentration in MD Simulation.Biophysical journal · 2018Article
- Complex dynamics at the nanoscale in simple biomembranes.Scientific reports · 2017Article
- Effects of Low Concentrations of Docosahexaenoic Acid on the Structure and Phase Behavior of Model Lipid Membranes.Membranes · 2015Article
- Improved Coarse-Grained Modeling of Cholesterol-Containing Lipid Bilayers.Journal of chemical theory and computation · 2014Article
- Core-based lipid nanoparticles as a nanoplatform for delivery of near-infrared fluorescent imaging agents.American journal of nuclear medicine and molecular imaging · 2014Article
- Modification of Lipid Bilayer Structure by Diacylglycerol: A Comparative Study of Diacylglycerol and Cholesterol.Journal of chemical theory and computation · 2012Article
- A statistical mechanical model of cholesterol/phospholipid mixtures: linking condensed complexes, superlattices, and the phase diagram.Journal of the American Chemical Society · 2012Article
- Orientation of tie-lines in the phase diagram of DOPC/DPPC/cholesterol model biomembranes.Langmuir : the ACS journal of surfaces and colloids · 2010Article
- Acyl-chain mismatch driven superlattice arrangements in DPPC/DLPC/cholesterol bilayers.The journal of physical chemistry. B · 2010Article
- A molecular view of the cholesterol condensing effect in DOPC lipid bilayers.The journal of physical chemistry. B · 2010Article
- Fluorescent analogs of biomolecular building blocks: design, properties, and applications.Chemical reviews · 2010Review
- Instability of cholesterol clusters in lipid bilayers and the cholesterol's Umbrella effect.The journal of physical chemistry. B · 2010Article
- Model membrane thermodynamics and lateral distribution of cholesterol: from experimental data to Monte Carlo simulation.Methods in enzymology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lateral organization of cholesterol in dioleoyl-phosphatidylcholine (DOPC) lipid bilayers at high cholesterol concentration (>45 mol%) was investigated using steady-state fluorescence anisotropy and fluorescent resonance energy transfer techniques. The recently devised Low Temperature Trap method was used to prepare compositionally uniform cholesterol/DOPC liposomes to avoid the problem of lipid demixing. The fluorescence anisotropy of diphenylhexatrience chain-labeled phosphatidylcholine (DPH-PC) in these liposomes exhibited local maxima at cholesterol mol fractions of 0.50 and 0.57, and a sharp drop at 0.67. For the liposomes labeled with both dehydroergosterol and DPH-PC, the fluorescent resonance energy transfer efficiency from dehydroergosterol to DPH-PC displayed a steep jump at cholesterol mol fraction of 0.5, and dips at 0.57 and 0.68. These results indicate the presence of highly ordered cholesterol regular distribution domains at those observed critical compositions. The observed critical mol fraction at 0.67 agreed favorably with the solubility limit of cholesterol in DOPC bilayers as independently measured by light scattering and optical microscopy. The regular distribution at 0.57 was previously predicted from a Monte Carlo simulation based on the Umbrella model. The results strongly support the hypothesis that the primary requirement for cholesterol-phospholipid mixing is that the polar phospholipid headgroups need to cover the nonpolar body of cholesterol to avoid the exposure of cholesterol to water.
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