ArticleBiophysical journal2004
Altered elementary calcium release events and enhanced calcium release by thymol in rat skeletal muscle.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Allosteric interactions between RyR channels justify intracellular Ca2+ release of skeletal muscle in quantitative detail.The Journal of general physiology · 2026Article
- Identification of Potential Human Ryanodine Receptor 1 Agonists and Molecular Mechanisms of Natural Small-Molecule Phenols as Anxiolytics.ACS omega · 2021Article
- Pharmacological Properties and Molecular Mechanisms of Thymol: Prospects for Its Therapeutic Potential and Pharmaceutical Development.Frontiers in pharmacology · 2017Review
- Effects of fluvastatin and coenzyme Q10 on skeletal muscle in normo- and hypercholesterolaemic rats.Journal of muscle research and cell motility · 2015Article
- Hypermuscular mice with mutation in the myostatin gene display altered calcium signalling.The Journal of physiology · 2014Article
- Characterization of functional TRPV1 channels in the sarcoplasmic reticulum of mouse skeletal muscle.PloS one · 2013Article
- Differential effects of phosphatase inhibitors on the calcium homeostasis and migration of HaCaT keratinocytes.PloS one · 2013Article
- Trisk 32 regulates IP(3) receptors in rat skeletal myoblasts.Pflugers Archiv : European journal of physiology · 2011Article
- Phenol increases intracellular [Ca2+] during twitch contractions in intact Xenopus skeletal myofibers.Journal of applied physiology (Bethesda, Md. : 1985) · 2010Article
- Altered expression of triadin 95 causes parallel changes in localized Ca2+ release events and global Ca2+ signals in skeletal muscle cells in culture.The Journal of physiology · 2008Article
- Effects of K-201 on the calcium pump and calcium release channel of rat skeletal muscle.Pflugers Archiv : European journal of physiology · 2008Article
- Charged surface area of maurocalcine determines its interaction with the skeletal ryanodine receptor.Biophysical journal · 2008Article
- Alterations in the calcium homeostasis of skeletal muscle from postmyocardial infarcted rats.Pflugers Archiv : European journal of physiology · 2007Article
- Sparks and embers of skeletal muscle: the exciting events of contractile activation.Pflugers Archiv : European journal of physiology · 2007Review
- Effect of natural phenol derivatives on skeletal type sarcoplasmic reticulum Ca2+ -ATPase and ryanodine receptor.Journal of muscle research and cell motility · 2007Article
- Differential effects of maurocalcine on Ca2+ release events and depolarization-induced Ca2+ release in rat skeletal muscle.The Journal of physiology · 2005Article
- The elementary events of Ca2+ release elicited by membrane depolarization in mammalian muscle.The Journal of physiology · 2004Article
- Voltage-activated elementary calcium release events in isolated mouse skeletal muscle fibers.The Journal of membrane biologyArticle
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effects of thymol on steps of excitation-contraction coupling were studied on fast-twitch muscles of rodents. Thymol was found to increase the depolarization-induced release of calcium from the sarcoplasmic reticulum, which could not be attributed to a decreased calcium-dependent inactivation of calcium release channels/ryanodine receptors or altered intramembrane charge movement, but rather to a more efficient coupling of depolarization to channel opening. Thymol increased ryanodine binding to heavy sarcoplasmic reticulum vesicles, with a half-activating concentration of 144 micro M and a Hill coefficient of 1.89, and the open probability of the isolated and reconstituted ryanodine receptors, from 0.09 +/- 0.03 to 0.22 +/- 0.04 at 30 micro M. At higher concentrations the drug induced long-lasting open events on a full conducting state. Elementary calcium release events imaged using laser scanning confocal microscopy in the line-scan mode were reduced in size, 0.92 +/- 0.01 vs. 0.70 +/- 0.01, but increased in duration, 56 +/- 1 vs. 79 +/- 1 ms, by 30 micro M thymol, with an increase in the relative proportion of lone embers. Higher concentrations favored long events, resembling embers in control, with duration often exceeding 500 ms. These findings provide direct experimental evidence that the opening of a single release channel will generate an ember, rather than a spark, in mammalian skeletal muscle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.