ArticleProceedings of the National Academy of Sciences of the United States of America2004
Microscopic observations reveal that fusogenic peptides induce liposome shrinkage prior to membrane fusion.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Analysis of Inner Content and Phospholipid Membrane Fusion during Two Step Freezing Induced Liposome Fusion.ACS omega · 2026Article
- Composition dependent effects of charged lipids on freeze-thaw induced fusion in dioleoyl phospholipid membranes.Biophysics and physicobiology · 2026Article
- Invagination of Giant Unilamellar Vesicles upon Membrane Mixing with Native Vesicles.ACS omega · 2024Article
- Article
- Constructing Smart Protocells with Built-In DNA Computational Core to Eliminate Exogenous Challenge.Journal of the American Chemical Society · 2018Article
- Tuning Liposome Membrane Permeability by Competitive Peptide Dimerization and Partitioning-Folding Interactions Regulated by Proteolytic Activity.Scientific reports · 2016Article
- Engineering protocells: prospects for self-assembly and nanoscale production-lines.Life (Basel, Switzerland) · 2015Review
- Effects of lipid composition and solution conditions on the mechanical properties of membrane vesicles.Membranes · 2015Article
- Divided we stand: splitting synthetic cells for their proliferation.Systems and synthetic biology · 2014Review
- Dynamic Morphological Changes Induced By GM1 and Protein Interactions on the Surface of Cell-Sized Liposomes.Materials (Basel, Switzerland) · 2013Article
- Multiple membrane interactions and versatile vesicle deformations elicited by melittin.Toxins · 2013Article
- Effect of repetitive lysine-tryptophan motifs on the bactericidal activity of antimicrobial peptides.Amino acids · 2013Article
- Unusual titration of the membrane-bound artificial hemagglutinin fusion peptide.European biophysics journal : EBJ · 2012Article
- A comparative evaluation of coenzyme Q10-loaded liposomes and solid lipid nanoparticles as dermal antioxidant carriers.International journal of nanomedicine · 2012Article
- Reversible liposome association induced by LAH4: a peptide with potent antimicrobial and nucleic acid transfection activities.Biophysical journal · 2010Article
- Fusion peptide from influenza hemagglutinin increases membrane surface order: an electron-spin resonance study.Biophysical journal · 2009Article
- Membrane insertion of the three main membranotropic sequences from SARS-CoV S2 glycoprotein.Biochimica et biophysica acta · 2008Article
- Interaction of lipopolysaccharide and phospholipid in mixed membranes: solid-state 31P-NMR spectroscopic and microscopic investigations.Biophysical journal · 2008Article
- SNAREpin/Munc18 promotes adhesion and fusion of large vesicles to giant membranes.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Triggering and visualizing the aggregation and fusion of lipid membranes in microfluidic chambers.Biophysical journal · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the mechanisms involved in membrane fusion, we visualized the fusion process of giant liposomes in real time by optical dark-field microscopy. To induce membrane fusion, we used (i) influenza hemagglutinin peptide (HA), a 20-aa peptide derived from the N-terminal fusion peptide region of the HA2 subunit, and (ii) two synthetic analogue peptides of HA, a negatively (E5) and positively (K5) charged analogue. We were able to visualize membrane fusion caused by E5 or by K5 alone, as well as by the mixture of these two peptides. The HA peptide however, did not induce membrane fusion, even at an acidic pH, which has been described as the optimal condition for the fusion of large unilamellar vesicles. Surprisingly, before membrane fusion, the shrinkage of liposomes was always observed. Our results suggest that a perturbation of lipid bilayers, which probably resulted from alterations in the bending folds of membranes, is a critical factor in fusion efficiency.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.