ArticlePlant physiology2004
Geminating pollen has tubular vacuoles, displays highly dynamic vacuole biogenesis, and requires VACUOLESS1 for proper function.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Autophagy and its mediated mitochondrial quality control maintain pollen tube growth and male fertility in Arabidopsis.Autophagy · 2023Article
- At the Nexus between Cytoskeleton and Vacuole: How Plant Cytoskeletons Govern the Dynamics of Large Vacuoles.International journal of molecular sciences · 2023Review
- Arabidopsis HOPS subunit VPS41 carries out plant-specific roles in vacuolar transport and vegetative growth.Plant physiology · 2022Article
- Molecular mechanisms of endomembrane trafficking in plants.The Plant cell · 2022Review
- VPS18-regulated vesicle trafficking controls the secretion of pectin and its modifying enzyme during pollen tube growth in Arabidopsis.The Plant cell · 2021Article
- New opportunities and insights into Papaver self-incompatibility by imaging engineered Arabidopsis pollen.Journal of experimental botany · 2020Article
- The mitochondrial NADThe Plant journal : for cell and molecular biology · 2019Article
- Vacuolar membrane structures and their roles in plant-pathogen interactions.Plant molecular biology · 2019Review
- Self-incompatibility in Papaver pollen: programmed cell death in an acidic environment.Journal of experimental botany · 2019Review
- Functional Disruption of the Tomato Putative Ortholog ofFrontiers in plant science · 2019Article
- Quantitative Proteome Analysis Reveals Changes in the Protein Landscape During Grape Berry Development With a Focus on Vacuolar Transport Proteins.Frontiers in plant science · 2019Article
- Microtubules play a role in trafficking prevacuolar compartments to vacuoles in tobacco pollen tubes.Open biology · 2018Article
- Phosphoinositides control the localization of HOPS subunit VPS41, which together with VPS33 mediates vacuole fusion in plants.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- ArabidopsisPlant physiology · 2018Article
- The ADAPTOR PROTEIN-3 Complex Mediates Pollen Tube Growth by Coordinating Vacuolar Targeting and Organization.Plant physiology · 2018Article
- Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases mediate membrane fusion at the vacuole in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Vacuolar trafficking in pollen tube growth and guidance.Plant signaling & behavior · 2018Review
- Efficient preparation of Arabidopsis pollen tubes for ultrastructural analysis using chemical and cryo-fixation.BMC plant biology · 2017Article
- Adaptor Protein-3-Dependent Vacuolar Trafficking Involves a Subpopulation of COPII and HOPS Tethering Proteins.Plant physiology · 2017Article
- Male functions and malfunctions: the impact of phosphoinositides on pollen development and pollen tube growth.Plant reproduction · 2016Review
1 more citing papers are in PubMed but not listed here.
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5 authors.
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Abstract
Vacuoles perform multiple functions in plants, and VCL1 (VACUOLESS1) is essential for biogenesis with loss of expression in the vcl1 mutant leading to lethality. Vacuole biogenesis plays a prominent role in gametophytes, yet is poorly understood. Given the importance of VCL1, we asked if it contributes to vacuole biogenesis during pollen germination. To address this question, it was essential to first understand the dynamics of vacuoles. A tonoplast marker, delta-TIP::GFP, under a pollen-specific promoter permitted the examination of vacuole morphology in germinating pollen of Arabidopsis. Our results demonstrate that germination involves a complex, yet definable, progression of vacuole biogenesis. Pollen vacuoles are extremely dynamic with remarkable features such as elongated (tubular) vacuoles and highly mobile cytoplasmic invaginations. Surprisingly, vcl1 did not adversely impact vacuole morphology in pollen germinated in vitro. To focus further on VCL1 in pollen, reciprocal backcrosses demonstrated reduced transmission of vcl1 through male gametophytes, indicating that vcl1 was expressive after germination. Interestingly, vcl1 affected the fertility of female gametophytes that undergo similarly complex vacuole biogenesis. Our results indicate that vcl1 is lethal in the sporophyte but is not fully expressive in the gametophytes. They also point to the complexity of pollen vacuoles and suggest that the mechanism of vacuole biogenesis in pollen may differ from that in other plant tissues.
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