ArticlePlant physiology2004
The seeds of Lotus japonicus lines transformed with sense, antisense, and sense/antisense galactomannan galactosyltransferase constructs have structurally altered galactomannans in their endosperm cell walls.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 52 citations in OpenAlex.
- The Plant Cell Wall: From Structural Diversity and Biosynthesis to Integrity Signaling and Physiological Functions.Plants (Basel, Switzerland) · 2026Review
- Transcriptomic, cytological, and physiological analyses reveal the potential regulatory mechanism in Tartary buckwheat under cadmium stress.Frontiers in plant science · 2022Article
- Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach.Scientific reports · 2021Article
- The synthesis of xyloglucan, an abundant plant cell wall polysaccharide, requires CSLC function.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Engineering Non-cellulosic Polysaccharides of Wood for the Biorefinery.Frontiers in plant science · 2018Article
- Article
- Deep EST profiling of developing fenugreek endosperm to investigate galactomannan biosynthesis and its regulation.Plant molecular biology · 2012Article
- Large-scale proteome comparative analysis of developing rhizomes of the ancient vascular plant equisetum hyemale.Frontiers in plant science · 2012Article
- Characterization of the mannan synthase promoter from guar (Cyamopsis tetragonoloba).Plant cell reports · 2011Article
- Regulation of seed germination in the close Arabidopsis relative Lepidium sativum: a global tissue-specific transcript analysis.Plant physiology · 2011Article
- Heterogeneity in the chemistry, structure and function of plant cell walls.Nature chemical biology · 2010Review
- Metabolic pathways in tropical dicotyledonous albuminous seeds: Coffea arabica as a case study.The New phytologist · 2009Article
- Characterization and expression analysis of genes directing galactomannan synthesis in coffee.Annals of botany · 2008Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Galactomannan biosynthesis in legume seed endosperms involves two Golgi membrane-bound glycosyltransferases, mannan synthase and galactomannan galactosyltransferase (GMGT). GMGT specificity is an important factor regulating the distribution and amount of (1-->6)-alpha-galactose (Gal) substitution of the (1-->4)-beta-linked mannan backbone. The model legume Lotus japonicus is shown now to have endospermic seeds with endosperm cell walls that contain a high-Gal galactomannan (mannose [Man]/Gal = 1.2-1.3). Galactomannan biosynthesis in developing L. japonicus endosperms has been mapped, and a cDNA encoding a functional GMGT has been obtained from L. japonicus endosperms during galactomannan deposition. L. japonicus has been transformed with sense, antisense, and sense/antisense ("hairpin loop") constructs of the GMGT cDNA. Some of the sense, antisense, and sense/antisense transgenic lines exhibited galactomannans with altered (higher) Man/Gal values in their (T(1) generation) seeds, at frequencies that were consistent with posttranscriptional silencing of GMGT. For T(1) generation individuals, transgene inheritance was correlated with galactomannan composition and amount in the endosperm. All the azygous individuals had unchanged galactomannans, whereas those that had inherited a GMGT transgene exhibited a range of Man/Gal values, up to about 6 in some lines. For Man/Gal values up to 4, the results were consistent with lowered Gal substitution of a constant amount of mannan backbone. Further lowering of Gal substitution was accompanied by a slight decrease in the amount of mannan backbone. Microsomal membranes prepared from the developing T(2) generation endosperms of transgenic lines showed reduced GMGT activity relative to mannan synthase. The results demonstrate structural modification of a plant cell wall polysaccharide by designed regulation of a Golgi-bound glycosyltransferase.
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