ArticleMolecular genetics and genomics : MGG2004
Molecular cloning of a cytosolic ascorbate peroxidase cDNA from cell cultures of sweet potato and its expression in response to stress.
Article in Molecular genetics and genomics : MGG, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 74 citations in OpenAlex.
- Halotolerant endophytic bacteria alleviate salinity stress in rice (oryza sativa L.) by modulating ion content, endogenous hormones, the antioxidant system and gene expression.BMC plant biology · 2023Article
- Achieving abiotic stress tolerance in plants through antioxidative defense mechanisms.Frontiers in plant science · 2023Review
- Ascorbate-Glutathione Cycle Genes Families in Euphorbiaceae: Characterization and Evolutionary Analysis.Biology · 2022Article
- Effects of Coir-Based Growing Medium with Municipal Solid Waste Compost or Biochar on Plant Growth, Mineral Nutrition, and Accumulation of Phytochemicals in Spinach.Plants (Basel, Switzerland) · 2022Article
- Characterization of APX and APX-R gene family in Brassica juncea and B. rapa for tolerance against abiotic stresses.Plant cell reports · 2022Article
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- Cloning and overexpression of the ascorbate peroxidase gene from the yam (Dioscorea alata) enhances chilling and flood tolerance in transgenic Arabidopsis.Journal of plant research · 2019Article
- Abiotic Stress Tolerance in Plants: Myriad Roles of Ascorbate Peroxidase.Frontiers in plant science · 2017Review
- Catalase and ascorbate peroxidase-representative H2O2-detoxifying heme enzymes in plants.Environmental science and pollution research international · 2016Review
- Abscisic Acid-Induced HFrontiers in plant science · 2016Article
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- Role of L-ascorbate in alleviating abiotic stresses in crop plants.Botanical studies · 2014Review
- Cloning and functional analysis of a novel ascorbate peroxidase (APX) gene from Anthurium andraeanum.Journal of Zhejiang University. Science. B · 2013Article
- Cloning, expression and functional validation of drought inducible ascorbate peroxidase (Ec-apx1) from Eleusine coracana.Molecular biology reports · 2013Article
- Plant responses to stresses: Role of ascorbate peroxidase in the antioxidant protection.Genetics and molecular biology · 2012Article
- Nitric oxide-activated calcium/calmodulin-dependent protein kinase regulates the abscisic acid-induced antioxidant defence in maize.Journal of experimental botany · 2012Article
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- Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress.Plant cell reports · 2010Article
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Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a cytosolic ascorbate peroxidase (APX), swAPX1, was isolated from cell cultures of sweet potato ( Ipomoea batatas) by cDNA library screening, and its expression in the context of various environmental stresses was investigated. swAPX1 contains an ORF of 250 amino acids (27.5 kDa) encoding a protein with a pI value of 5.32. The swAPX1 ORF does not code for a transit peptide, suggesting that the product is a cytosolic isoform. RNA blot analysis showed that swAPX1 gene is expressed in cultured cells and mature leaves, but not in stems, non-storage or storage roots of sweet potato. The level of swAPX1 RNA progressively increased during cell growth in suspension cultures. In leaf tissues, the gene responded differentially to various abiotic stresses, as revealed by RT-PCR analysis. swAPX1 was highly induced in leaves by wounding, and treatment with methyl viologen (50 microM), hydrogen peroxide (440 mM), abscisic acid (ABA; 100 microM) or exposure to high temperature (37 degrees C). In addition, the gene was strongly induced in the leaves following inoculation with a bacterial pathogen ( Pectobacterium chrysanthemi). These results indicate that swAPX1 may be involved in hydrogen peroxide-detoxification and thus help to overcome the oxidative stress induced by abiotic and biotic stresses.
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