ArticlePflugers Archiv : European journal of physiology2004
Releasing the peri-neuronal net to patch-clamp neurons in adult CNS.
Article in Pflugers Archiv : European journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Chondroitin 4-Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The Effect of Hapln4 Link Protein Deficiency on Extracellular Space Diffusion Parameters and Perineuronal Nets in the Auditory System During Aging.Neurochemical research · 2020Article
- Changes in membrane properties of rat deep cerebellar nuclear projection neurons during acquisition of eyeblink conditioning.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The extracellular matrix molecule brevican is an integral component of the machinery mediating fast synaptic transmission at the calyx of Held.The Journal of physiology · 2015Article
- Maturation of membrane properties of neurons in the rat deep cerebellar nuclei.Developmental neurobiology · 2014Article
- Retrograde fluorescent labeling allows for targeted extracellular single-unit recording from identified neurons in vivo.Journal of visualized experiments : JoVE · 2013Article
- Long-duration perforated patch recordings from spinal interneurons of adult mice.Journal of neurophysiology · 2011Article
- Dopamine Modulates Spike Timing-Dependent Plasticity and Action Potential Properties in CA1 Pyramidal Neurons of Acute Rat Hippocampal Slices.Frontiers in synaptic neuroscience · 2011Article
- High-threshold K+ current increases gain by offsetting a frequency-dependent increase in low-threshold K+ current.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extracellular matrix of adult neural tissue contains chondroitin sulphated proteogylcans that form a dense peri-neuronal net surrounding the cell body and proximal dendrites of many neuronal classes. Development of the peri-neuronal net beyond approximately postnatal day 17 obscures visualization and often access by patch electrodes to neuronal membranes with the result that patch clamp recordings are most readily obtained from early postnatal animals. We describe a technique in which the surface tension of a sucrose-based medium promotes partial dissociation of thin tissue slices from adult tissue. Surface tension spreads the tissue and loosens the peri-neuronal net from neuronal membranes within minutes and in the absence of proteolytic enzymes. Furthermore, the extent of dissociation can be controlled so as to maintain the overall slice structure and allow identification of specific cell classes. Excellent structural preservation of neurons and dendrites can be obtained and full access by patch electrodes made possible for current- or voltage-clamp recordings in tissue well beyond the development of peri-neuronal nets. We demonstrate the feasibility of using this approach through patch recordings from neurons in the brainstem and cerebellum of adult gymnotiform fish and in deep cerebellar nuclei of rats as old as 6 months.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.