ArticleGene therapy2004
Polyphosphoramidate gene carriers: effect of charge group on gene transfer efficiency.
Article in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Intrathecal drug delivery in the era of nanomedicine.Advanced drug delivery reviews · 2020Pooled it
- Dual-Functional Polyphosphoesters for Gene Delivery: Synergistic Effects of Guanidinium and Hydrophobic Side Chains in Degradable Polymers.Advanced healthcare materials · 2026Article
- Synthesis and Characterization of Amphiphilic Diblock Polyphosphoesters Containing Lactic Acid Units for Potential Drug Delivery Applications.Molecules (Basel, Switzerland) · 2023Article
- Cationic Nanomaterials for Autoimmune Diseases Therapy.Frontiers in pharmacology · 2021Review
- Opening up the Toolbox: Synthesis and Mechanisms of Phosphoramidates.Molecules (Basel, Switzerland) · 2020Review
- The effect and role of carbon atoms in poly(β-amino ester)s for DNA binding and gene delivery.Journal of the American Chemical Society · 2013Article
- Probing in vivo trafficking of polymer/DNA micellar nanoparticles using SPECT/CT imaging.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Article
- Nucleic acid-binding polymers as anti-inflammatory agents.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Biocleavable Polycationic Micelles as Highly Efficient Gene Delivery Vectors.Nanoscale research letters · 2010Article
- Hydrophilic/lipophilic N-methylene phosphonic chitosan as a promising non-viral vector for gene delivery.Journal of materials science. Materials in medicine · 2010Article
- Combinatorial evaluation of cations, pH-sensitive and hydrophobic moieties for polymeric vector design.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Quantitative comparison of intracellular unpacking kinetics of polyplexes by a model constructed from quantum dot-FRET.Molecular therapy : the journal of the American Society of Gene Therapy · 2008Article
- Nano-vectors for efficient liver specific gene transfer.International journal of nanomedicine · 2008Review
- PEG-b-PPA/DNA micelles improve transgene expression in rat liver through intrabiliary infusion.Journal of controlled release : official journal of the Controlled Release Society · 2007Article
- Optimizing targeted gene delivery: chemical modification of viral vectors and synthesis of artificial virus vector systems.The AAPS journal · 2006Review
- Chitosan-DNA nanoparticles delivered by intrabiliary infusion enhance liver-targeted gene delivery.International journal of nanomedicine · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cationic polymeric carriers have been widely used for gene delivery. However, the structure-function relationship, especially the effect of charge groups of cationic polymeric carriers on the transfection activity, is poorly understood. To examine this important parameter, a series of cationic polymers, polyphosphoramidates (PPAs) with an identical backbone, same side chain spacer, similar molecular weights but different charge groups containing primary to quaternary amino groups (PPA-EA, PPA-MEA, PPA-DMA and PPA-TMA, Figure 1) were synthesized. The DNA-binding affinity of these four PPAs increased in the order of PPA-EA<PPA-MEA<PPA-DMA approximately PPA-TMA. The cytotoxicity decreased in the order of PPA-EA>PPA-MEA>PPA-DMA>PPA-TMA. Particle size and zeta potential of four different types of PPA/DNA nanoparticles did not show significant correlation with PPA structure. These PPAs did not show significant buffering capacity within pH 5-7, even though transfection mediated by PPA-EA was the only one that seemed to be limited by endolysomal escape. Endocytosis of DNA mediated by PPAs was also similar (17-22%) for all four PPAs. However, the transfection efficiency of these PPAs varied significantly. In vitro transfection efficiency of PPAs decreased in the order of PPA-EA>PPA-MEA>PPA-DMA approximately PPA-TMA. Nanoparticles with PPA-EA containing primary amino groups gave the highest transfection efficiency in cell lines at the charge ratios from 6/1 to 20/1 (+/-). Matching the trend of transfection efficiency observed in vitro, PPA-EA mediated the highest transgene expression, comparable to that of polyethylenimine, in the spinal cord following intrathecal injection of the nanoparticles. These results establish that PPA gene carriers with primary amino group side chains are more potent than those with secondary, tertiary or quaternary amino groups in vitro and in the intrathecal gene delivery model.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.