ArticleGene therapy2004
Sea urchin insulator protects lentiviral vector from silencing by maintaining active chromatin structure.
Article in Gene therapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- A mouse model for the study of anti-tumor T cell responses in Kras-driven lung adenocarcinoma.Cell reports methods · 2021Article
- Following the adverse outcome pathway from micronucleus to cancer using H2B-eGFP transgenic healthy stem cells.Archives of toxicology · 2020Article
- An insulator element located at the cyclin B1 interacting protein 1 gene locus is highly conserved among mammalian species.PloS one · 2015Article
- DNA methylation and histone modifications are the molecular lock in lentivirally transduced hematopoietic progenitor cells.BioMed research international · 2015Article
- Article
- Highly efficient generation of transgenic sheep by lentivirus accompanying the alteration of methylation status.PloS one · 2013Article
- Article
- Daedalus: a robust, turnkey platform for rapid production of decigram quantities of active recombinant proteins in human cell lines using novel lentiviral vectors.Nucleic acids research · 2011Article
- The use of chromatin insulators to improve the expression and safety of integrating gene transfer vectors.Human gene therapy · 2011Review
- Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia.Stem cell research & therapy · 2011Article
- The GATA1-HS2 enhancer allows persistent and position-independent expression of a β-globin transgene.PloS one · 2011Article
- Ex vivo transduction and transplantation of bone marrow cells for liver gene delivery of alpha1-antitrypsin.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- BAC TG-EMBED: one-step method for high-level, copy-number-dependent, position-independent transgene expression.Nucleic acids research · 2010Article
- RNA interference-based therapeutics for human immunodeficiency virus HIV-1 treatment: synthetic siRNA or vector-based shRNA?Expert opinion on biological therapy · 2010Review
- In vivo expression of MHC class I genes depends on the presence of a downstream barrier element.PloS one · 2009Article
- The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing.Journal of virology · 2008Article
- In vivo selection of genetically modified erythroblastic progenitors leads to long-term correction of beta-thalassemia.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Gene delivery by lentivirus vectors.Molecular biotechnology · 2007Review
- Ars insulator protects transgenes from long-term silencing in sea urchin larva.Development genes and evolution · 2007Article
- Dynamic DNA methylation and histone modifications contribute to lentiviral transgene silencing in murine embryonic carcinoma cells.Journal of virology · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Suppressed expression of transgenes in vivo is the major obstacle in the gene therapy. For the long-term expression, we utilized a chromatin insulator from sea urchin arylsulfatase (Ars) gene locus (Ars insulator, ArsI), which has been shown to epigenetically regulate gene expression across species. ArsI was able to prevent silencing of the transgene in a myeloid cell line, HL-60, and a murine embryonic stem cell line, CCE, in an orientation-dependent manner, but not in Huh-7, K562 and MCF-7 cells, indicating that the effect of ArsI on gene silencing was cell type dependent. Although anti-silencing effect of ArsI was almost equivalent to that of chicken beta-globin insulator, incorporation of ArsI into lentiviral vector had little effect on the virus titer compared with chicken beta-globin insulator. Clonal analysis of transduced HL-60 cells revealed that ArsI protects the lentiviral vector from position effects regardless of its orientation. Furthermore, chromatin immunoprecipitation assays revealed that a high acetylation level was observed in the promoter of the insulated vector, whereas that of ArsI was independent of its anti-silencing capacity. In addition to it having little deteriorative effect on the virus titer, the identified anti-silencing effect of ArsI suggested its possibility for application in gene therapy.
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