ArticleProceedings of the National Academy of Sciences of the United States of America2004
Annexin 2-caveolin 1 complex is a target of ezetimibe and regulates intestinal cholesterol transport.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 134 citations in OpenAlex.
- Ezetimibe suppresses cholesterol accumulation in lipid-loaded vascular smooth muscle cells in vitro via MAPK signaling.Acta pharmacologica Sinica · 2014Article
- Article
- Automated in vivo platform for the discovery of functional food treatments of hypercholesterolemia.PloS one · 2013Article
- Emerging applications for zebrafish as a model organism to study oxidative mechanisms and their roles in inflammation and vascular accumulation of oxidized lipids.Free radical biology & medicine · 2012Review
- Cyclophilin A and viral infections.Biochemical and biophysical research communications · 2012Review
- Ezetimibe therapy: mechanism of action and clinical update.Vascular health and risk management · 2012Review
- Cholesterol transport from late endosomes to the Golgi regulates t-SNARE trafficking, assembly, and function.Molecular biology of the cell · 2011Article
- Cannabinoid receptor signalling in neurodegenerative diseases: a potential role for membrane fluidity disturbance.British journal of pharmacology · 2011Review
- Lessons from morpholino-based screening in zebrafish.Briefings in functional genomics · 2011Review
- Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport.Annual review of physiology · 2011Review
- A novel model of cholesterol efflux from lipid-loaded cells.Acta pharmacologica Sinica · 2010Review
- NPC1L1 and cholesterol transport.FEBS letters · 2010Review
- Protein mediators of sterol transport across intestinal brush border membrane.Sub-cellular biochemistry · 2010Review
- The annexins: spatial and temporal coordination of signaling events during cellular stress.Cellular and molecular life sciences : CMLS · 2009Review
- Vascular lipid accumulation, lipoprotein oxidation, and macrophage lipid uptake in hypercholesterolemic zebrafish.Circulation research · 2009Article
- A primer for morpholino use in zebrafish.Zebrafish · 2009Review
- Extracellular loop C of NPC1L1 is important for binding to ezetimibe.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Wolman disease/cholesteryl ester storage disease: efficacy of plant-produced human lysosomal acid lipase in mice.Journal of lipid research · 2008Article
- The role of Niemann-Pick C1 - Like 1 (NPC1L1) in intestinal sterol absorption.Journal of clinical lipidology · 2008Article
- Annexin A6-induced alterations in cholesterol transport and caveolin export from the Golgi complex.Traffic (Copenhagen, Denmark) · 2007Article
Corrections and comments
- Retraction · 2012-12-24Falsification/Fabrication of Image · Investigation by ORI · Misconduct - Official Investigation(s) and/or Finding(s) · Misconduct by Author ·
- Commented on by
- Commented on by
- Retracted
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Modulation of cholesterol absorption in the intestine, the primary site of dietary cholesterol uptake in humans, can have profound clinical implications. We have undertaken a reverse genetic approach by disrupting putative cholesterol processing genes in zebrafish larvae by using morpholino (MO) antisense oligonucleotides. By using targeted MO injections and immunoprecipitation (IP) experiments coupled with mass spectrometry, we determined that annexin (ANX)2 complexes with caveolin (CAV)1 in the zebrafish and mouse intestine. The complex is heat stable and unaffected by SDS or reducing conditions. MO targeting of anx2b or cav1, which are both strongly expressed in the larval and adult zebrafish intestinal epithelium, prevents formation of the protein heterocomplex. Furthermore, anx2b MO injection prevents processing of a fluorescent cholesterol reporter and results in reduced sterol mass. Pharmacological treatment of mice with ezetimibe disrupts the heterocomplex in only hypercholesterolemic animals. These data suggest that ANX2 and CAV1 are components of an intestinal sterol transport complex.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.