ArticleNucleic acids research2004
A high-throughput method to monitor the expression of microRNA precursors.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 187 papers.
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Who cites it
187 citing papers in PubMed, 481 citations in OpenAlex.
- Protocol for high-sensitivity detection of microRNAs in adult Drosophila tissues using hybridization chain reaction.STAR protocols · 2026Article
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- Quantification of EV-associated miRNA in liquid biopsies for biomarker signature development.Extracellular vesicles and circulating nucleic acids · 2026Review
- Noradrenergic activation of the basolateral amygdala facilitates memory specificity for similar events experienced close in time.Nature neuroscience · 2025Article
- Ythdf2 facilitates precursor miR-378/miR-378-5p maturation to support myogenic differentiation.Cellular and molecular life sciences : CMLS · 2024Article
- Loss of miR-634 contributes to the formation FOXA1-positive triple negative breast cancer subtype.Discover oncology · 2024Article
- Early Leishmania infectivity depends on miR-372/373/520d family-mediated reprogramming of polyamines metabolism in THP-1-derived macrophages.Scientific reports · 2024Article
- RNA mGenes & diseases · 2024Article
- Current strategies for early epithelial ovarian cancer detection using miRNA as a potential tool.Frontiers in molecular biosciences · 2024Review
- LncRNA miR663AHG represses the development of colon cancer in a miR663a-dependent manner.Cell death discovery · 2023Article
- MicroRNAs as clinical tools for diagnosis, prognosis, and therapy in prostate cancer.Translational oncology · 2023Article
- The miR-532-E2F1 feedback loop contributes to gastric cancer progression.Cell death & disease · 2022Article
- OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression.Nucleic acids research · 2022Article
- Expression Profiles of Estrogen-Regulated MicroRNAs in Cancer Cells.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Experimental MicroRNA Targeting Validation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Characterization and Anticancer Activity of Biosynthesized Au/Cellulose Nanocomposite fromPolymers · 2021Article
- Mirna Expression in Glaucomatous and TGFβ2 Treated Lamina Cribrosa Cells.International journal of molecular sciences · 2021Article
- Recent trends in application of nanomaterials for the development of electrochemical microRNA biosensors.Mikrochimica acta · 2021Review
- miRNA Targets: From Prediction Tools to Experimental Validation.Methods and protocols · 2020Review
127 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
microRNAs (miRNAs) are small, functional, non-coding RNAs. miRNAs are transcribed as long primary transcripts (primary precursors) that are processed to the approximately 75 nt precursors (pre-miRNAs) by the nuclear enzyme Drosha. The approximately 22 nt mature miRNA is processed from the pre-miRNA by the RNase III Dicer. The vast majority of published studies to date have used northern blotting to detect the expression of miRNAs. We describe here a sensitive, high throughput, real-time PCR assay to monitor the expression of miRNA precursors. Gene-specific primers and reverse transcriptase were used to convert the primary precursors and pre-miRNAs to cDNA. The expression of 23 miRNA precursors in six human cancer cell lines was assayed using the PCR assay. The miRNA precursors accumulated to different levels when compared with each other or when a single precursor is compared in the various cell lines. The precursor expression profile of three miRNAs determined by the PCR assay was identical to the mature miRNA expression profile determined by northern blotting. We propose that the PCR assay may be scaled up to include all of the 150+ known human miRNA genes and can easily be adaptable to other organisms such as plants, Caenorhabditis elegans and Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.