ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Enhanced relative expression of glutamate receptor 1 flip AMPA receptor subunits in hippocampal astrocytes of epilepsy patients with Ammon's horn sclerosis.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 85 citations in OpenAlex.
- Analysis of the Immunoinflammatory Microenvironment Characteristics of Epilepsy Based on Single-Cell Transcriptomics.Journal of inflammation research · 2026Review
- Astrocytes in the initiation and progression of epilepsy.Nature reviews. Neurology · 2022Review
- Synaptic Reshaping and Neuronal Outcomes in the Temporal Lobe Epilepsy.International journal of molecular sciences · 2021Review
- Perampanel as first add-on choice on the treatment of mesial temporal lobe epilepsy: an observational real-life study.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2021Observational
- Ionotropic Glutamate Receptors in Epilepsy: A Review Focusing on AMPA and NMDA Receptors.Biomolecules · 2020Review
- Astrocytes as Guardians of Neuronal Excitability: Mechanisms Underlying Epileptogenesis.Frontiers in neurology · 2020Review
- R/G editing in GluA2RScientific reports · 2017Article
- Glia-neuron interactions in neurological diseases: Testing non-cell autonomy in a dish.Brain research · 2017Review
- Astrocyte uncoupling as a cause of human temporal lobe epilepsy.Brain : a journal of neurology · 2015Article
- Contributions of astrocytes to epileptogenesis following status epilepticus: opportunities for preventive therapy?Neurochemistry international · 2013Review
- Methodological limitations in determining astrocytic gene expression.Frontiers in endocrinology · 2013Review
- Review
- Astroglial networks and implications for therapeutic neuromodulation of epilepsy.Frontiers in computational neuroscience · 2012Article
- Alternatively spliced three novel transcripts of gria1 in the cerebellum and cortex of mouse brain.Neurochemical research · 2012Article
- Roles of NG2 glial cells in diseases of the central nervous system.Neuroscience bulletin · 2011Review
- Precursor cell biology and the development of astrocyte transplantation therapies: lessons from spinal cord injury.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2011Review
- Neuroglial roots of neurodegenerative diseases?Molecular neurobiology · 2011Review
- Astrocytes and epilepsy.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2010Review
- Neuron-glia synapses in the brain.Brain research reviews · 2010Review
- Three-dimensional relationships between perisynaptic astroglia and human hippocampal synapses.Glia · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Astrocytes express ionotropic glutamate receptors (GluRs), and recent evidence suggests that these receptors contribute to direct signaling between neurons and glial cells in vivo. Here, we have used functional and molecular analyses to investigate receptor properties in astrocytes of human hippocampus resected from patients with pharmacoresistant temporal lobe epilepsy (TLE). Histopathological analysis allowed us to distinguish two forms of epilepsy: Ammon's horn sclerosis (AHS) and lesion-associated TLE. Human hippocampal astrocytes selectively expressed the AMPA subtype of ionotropic glutamate receptors. Single-cell RT-PCR found preferential expression of the subunits GluR1 and GluR2 in human astrocytes, and the expression patterns were similar in patients with AHS and lesion-associated epilepsy. The AMPA receptor-specific modulators, cyclothiazide (CTZ) and 4-[2-(phenylsulfonylamino)ethylthio]-2,6-difluoro-phenoxyacetamide (PEPA), were used to investigate splice variant expression. Astrocytes of sclerotic specimens displayed a slower dissociation of CTZ from the receptor and a lower ratio of current potentiation by PEPA to potentiation by CTZ, suggesting enhanced expression of flip receptor variants in AHS versus lesion-associated epilepsy. Real-time PCR and restriction analysis substantiated this presumption by identifying elevated flip-to-flop mRNA ratios of GluR1 in single astrocytes of AHS specimens. These findings imply that in AHS, glutamate may lead to prolonged depolarization of astrocytes, thereby facilitating the generation or spread of seizure activity.
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