ArticleThe Biochemical journal2004
Determination of cellular nicotinic acid-adenine dinucleotide phosphate (NAADP) levels.
Article in The Biochemical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- NAADP-Mediated CaHandbook of experimental pharmacology · 2023Article
- NAADP-binding proteins find their identity.Trends in biochemical sciences · 2022Review
- Essential requirement for JPT2 in NAADP-evoked CaScience signaling · 2021Article
- NAADP Receptors.Cold Spring Harbor perspectives in biology · 2019Review
- Two-pore channels and disease.Biochimica et biophysica acta. Molecular cell research · 2018Review
- Ruling out pyridine dinucleotides as true TRPM2 channel activators reveals novel direct agonist ADP-ribose-2'-phosphate.The Journal of general physiology · 2015Article
- NADPH-generating systems in bacteria and archaea.Frontiers in microbiology · 2015Review
- Cyclic ADP-Ribose and NAADP in Vascular Regulation and Diseases.Messenger (Los Angeles, Calif. : Print) · 2013Article
- Bidirectional Ca²⁺ signaling occurs between the endoplasmic reticulum and acidic organelles.The Journal of cell biology · 2013Article
- Nicotinic acid adenine dinucleotide phosphate (NAADP) degradation by alkaline phosphatase.The Journal of biological chemistry · 2012Article
- The role of CD38 in Fcγ receptor (FcγR)-mediated phagocytosis in murine macrophages.The Journal of biological chemistry · 2012Article
- Identification of direct and indirect effectors of the transient receptor potential melastatin 2 (TRPM2) cation channel.The Journal of biological chemistry · 2010Article
- The calcium-mobilizing messenger nicotinic acid adenine dinucleotide phosphate participates in sperm activation by mediating the acrosome reaction.The Journal of biological chemistry · 2010Article
- Two-pore channels: Regulation by NAADP and customized roles in triggering calcium signals.Cell calcium · 2010Review
- Refinement of a radioreceptor binding assay for nicotinic acid adenine dinucleotide phosphate.Analytical biochemistry · 2007Article
- Ca2+ release triggered by NAADP in hepatocyte microsomes.The Biochemical journal · 2006Article
- NAADP binding to its target protein in sea urchin eggs requires phospholipids.The Biochemical journal · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nicotinic acid-adenine dinucleotide phosphate (NAADP) is fast emerging as a new intracellular Ca2+-mobilizing messenger. In sea urchin egg homogenates, binding of NAADP to its receptor is not readily reversible; hence, prior incubation with low concentrations of NAADP is more effective in inhibiting subsequent binding of radiolabelled NAADP than incubating the preparation with the two ligands simultaneously [Patel, Churchill and Galione (2000) Biochem. J. 352, 725-729]. We extend this finding to show that NAADP is more effective still in inhibiting the subsequent radioligand binding at lower homogenate concentrations, an effect again quite probably due to the non-reversible nature of the receptor-ligand interaction. Enhanced sensitivity of the preparation to NAADP afforded by simple manipulation of the experimental conditions has been applied to determine low levels of NAADP in acid extracts from human red blood cells, rat hepatocytes and Escherichia coli without interference from NADP breakdown. Our improved method for the quantification of NAADP should prove useful in the further assessment of its signalling role within cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.