ArticleNucleic acids research2004
Homologous gene targeting in Caenorhabditis elegans by biolistic transformation.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 83 citations in OpenAlex.
- Downregulation of transposable elements extends lifespan in Caenorhabditis elegans.Nature communications · 2023Article
- A Decade of CRISPR-Cas Gnome Editing inInternational journal of molecular sciences · 2022Review
- Animal Models to Study MicroRNA Function.Advances in cancer research · 2017Review
- Rac1/RhoA antagonism defines cell-to-cell heterogeneity during epidermal morphogenesis in nematodes.The Journal of cell biology · 2016Article
- Spatial control of active CDC-42 during collective migration of hypodermal cells in Caenorhabditis elegans.Journal of molecular cell biology · 2016Article
- Review
- Article
- Culturing Caenorhabditis elegans in axenic liquid media and creation of transgenic worms by microparticle bombardment.Journal of visualized experiments : JoVE · 2014Article
- pix-1 controls early elongation in parallel with mel-11 and let-502 in Caenorhabditis elegans.PloS one · 2014Article
- Efficient genome editing in Caenorhabditis elegans by CRISPR-targeted homologous recombination.Nucleic acids research · 2013Article
- Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.Nature methods · 2013Article
- Efficient and rapid C. elegans transgenesis by bombardment and hygromycin B selection.PloS one · 2013Article
- Nucleic acid transfection and transgenesis in parasitic nematodes.Parasitology · 2012Review
- A pre-mRNA-associating factor links endogenous siRNAs to chromatin regulation.PLoS genetics · 2011Article
- Different Mi-2 complexes for various developmental functions in Caenorhabditis elegans.PloS one · 2010Article
- Reverse genetics in eukaryotes.Biology of the cell · 2010Review
- Generation of transgenic C. elegans by biolistic transformation.Journal of visualized experiments : JoVE · 2010Article
- skn-1-Dependent and -independent regulation of aip-1 expression following metabolic stress in Caenorhabditis elegans.Molecular and cellular biology · 2010Article
- Targeted gene deletions in C. elegans using transposon excision.Nature methods · 2010Article
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Targeted homologous recombination is a powerful approach for genome manipulation that is widely used for gene alteration and knockouts in mouse and yeast. In Caenorhabditis elegans, several methods of target-selected mutagenesis have been implemented but none of them provides the opportunity of introducing exact predefined changes into the genome. Although anecdotal cases of homologous gene targeting in C.elegans have been reported, no practical technique of gene targeting has been developed so far. In this work we demonstrate that transformation of C.elegans by microparticle bombardment (biolistic transformation) can result in homologous recombination between introduced DNA and the chromosomal locus. We describe a scaled up version of biolistic transformation that can be used as a method for homologous gene targeting in the worm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.