ArticleNucleic acids research2004
The active form of Xp54 RNA helicase in translational repression is an RNA-mediated oligomer.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 121 citations in OpenAlex.
- Med9 Is a Novel eIF4E-Interacting Protein inInternational journal of molecular sciences · 2026Article
- Role of DEAD/DEAH-box helicases in immunity, infection and cancers.Cell communication and signaling : CCS · 2025Review
- Me31B: a key repressor in germline regulation and beyond.Bioscience reports · 2024Review
- Regulation and mechanisms of action of RNA helicases.RNA biology · 2024Review
- RNP components condense into repressive RNP granules in the aging brain.Nature communications · 2022Article
- Specific Interaction of DDX6 with an RNA Hairpin in the 3' UTR of the Dengue Virus Genome Mediates GJournal of virology · 2021Article
- The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity.iScience · 2020Article
- Numerous interactions act redundantly to assemble a tunable size of P bodies inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Translational repression of theRNA (New York, N.Y.) · 2017Article
- Article
- Mutational analysis of the RNA helicase Dhh1 in Ste12 expression and yeast mating.Journal of microbiology (Seoul, Korea) · 2017Article
- CPEB and miR-15/16 Co-Regulate Translation of Cyclin E1 mRNA during Xenopus Oocyte Maturation.PloS one · 2016Article
- Division of Labor in an Oligomer of the DEAD-Box RNA Helicase Ded1p.Molecular cell · 2015Article
- Xenopus CAF1 requires NOT1-mediated interaction with 4E-T to repress translation in vivo.RNA (New York, N.Y.) · 2015Article
- Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1.RNA (New York, N.Y.) · 2014Article
- An AU-rich instability element in the 3'UTR mediates an increase in mRNA stability in response to expression of a dhh1 ATPase mutant.Translation (Austin, Tex.) · 2014Article
- Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions.Nucleic acids research · 2013Article
- DEAD-box helicases as integrators of RNA, nucleotide and protein binding.Biochimica et biophysica acta · 2013Review
- The DHH1/RCKp54 family of helicases: an ancient family of proteins that promote translational silencing.Biochimica et biophysica acta · 2013Review
- Role of Rck-Pat1b binding in assembly of processing-bodies.RNA biology · 2013Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, we reported that in clam oocytes, cytoplasmic polyadenylation element-binding protein (CPEB) co-immunoprecipitates with p47, a member of the highly conserved RCK family of RNA helicases which includes Drosophila Me31B and Saccharomyces cerevisiae Dhh1. Xp54, the Xenopus homologue, with helicase activity, is a component of stored mRNP. In tethered function assays in Xenopus oocytes, we showed that MS2-Xp54 represses the translation of non-adenylated firefly luciferase mRNAs and that mutations in two core helicase motifs, DEAD and HRIGR, surprisingly, activated translation. Here we show that wild-type MS2-Xp54 tethered to the reporter mRNA 3'-untranslated region (UTR) represses translation in both oocytes and eggs in an RNA-dependent complex with endogenous Xp54. Injection of mutant helicases or adenylated reporter mRNA abrogates this association. Thus Xp54 oligomerization is a hallmark of translational repression. Xp54 complexes, which also contain CPEB and eIF4E in oocytes, change during meiotic maturation. In eggs, CPEB is degraded and, while eIF4E still interacts with Xp54, this interaction becomes RNA dependent. Supporting evidence for RNA-mediated oligomerization of endogenous Xp54, and RNA-independent association with CPEB and eIF4E in oocytes was obtained by gel filtration. Altogether, our data are consistent with a model in which the active form of the Xp54 RNA helicase is an oligomer in vivo which, when tethered, via either MS2 or CPEB to the 3'UTR, represses mRNA translation, possibly by sequestering eIF4E from the translational machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.