ArticleThe Journal of biological chemistry2004
Biochemical, crystallographic, and mutagenic characterization of hint, the AMP-lysine hydrolase, with novel substrates and inhibitors.
Article in The Journal of biological chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 61 citations in OpenAlex.
- Fast, accurate, and cost-effective poultry sex genotyping using real-time polymerase chain reaction.Frontiers in veterinary science · 2023Article
- Article
- The Enzyme-Free Release of Nucleotides from Phosphoramidates Depends Strongly on the Amino Acid.Angewandte Chemie (International ed. in English) · 2020Article
- Article
- Natural Products Containing 'Rare' Organophosphorus Functional Groups.Molecules (Basel, Switzerland) · 2019Review
- Crystal Structure of Histidine Triad Nucleotide-Binding Protein from the Pathogenic FungusMolecules and cells · 2019Article
- Axonal neuropathy with neuromyotonia: there is a HINT.Brain : a journal of neurology · 2017Review
- Kinetic mechanism of human histidine triad nucleotide binding protein 1.Biochemistry · 2013Article
- A new crystal form of human histidine triad nucleotide-binding protein 1 (hHINT1) in complex with adenosine 5'-monophosphate at 1.38 Å resolution.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Side chain independent recognition of aminoacyl adenylates by the Hint1 transcription suppressor.The journal of physical chemistry. B · 2012Article
- Histidine triad nucleotide-binding protein 1 (HINT-1) phosphoramidase transforms nucleoside 5'-O-phosphorothioates to nucleoside 5'-O-phosphates.The Journal of biological chemistry · 2010Article
- Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins.RNA (New York, N.Y.) · 2009Article
- Crystallographic and biochemical analysis of rotavirus NSP2 with nucleotides reveals a nucleoside diphosphate kinase-like activity.Journal of virology · 2007Article
- Hint2, a mitochondrial apoptotic sensitizer down-regulated in hepatocellular carcinoma.Gastroenterology · 2006Article
- New autosomal recessive cerebellar ataxias with oculomotor apraxia.Current neurology and neuroscience reports · 2005Review
- Disease-associated mutations inactivate AMP-lysine hydrolase activity of Aprataxin.The Journal of biological chemistry · 2005Article
- HinT proteins and their putative interaction partners in Mollicutes and Chlamydiaceae.BMC microbiology · 2005Article
- 31P NMR and genetic analysis establish hinT as the only Escherchia coli purine nucleoside phosphoramidase and as essential for growth under high salt conditions.The Journal of biological chemistry · 2005Article
- Altered specificity of Hint-W123Q supports a role for Hint inhibition by ASW in avian sex determination.Physiological genomics · 2004Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Hint, histidine triad nucleotide-binding protein, is a universally conserved enzyme that hydrolyzes AMP linked to lysine and, in yeast, functions as a positive regulator of the RNA polymerase II C-terminal domain kinase, Kin28. To explore the biochemical and structural bases for the adenosine phosphoramidate hydrolase activity of rabbit Hint, we synthesized novel substrates linking a p-nitroaniline group to adenylate (AMP-pNA) and inhibitors that consist of an adenosine group and 5'-sulfamoyl (AdoOSO(2)NH(2)) or N-ethylsulfamoyl (AdoOSO(2)NHCH(2)CH(3)) group. AMP-pNA is a suitable substrate for Hint that allowed characterization of the inhibitors; titration of each inhibitor into AMP-pNA assays revealed their K(i) values. The N-ethylsulfamoyl derivative has a 13-fold binding advantage over the sulfamoyl adenosine. The 1.8-A cocrystal structure of rabbit Hint with N-ethylsulfamoyl adenosine revealed a binding site for the ethyl group against Trp-123, a residue that reaches across the Hint dimer interface to interact with the alkyl portion of the inhibitor and, presumably, the alkyl portion of a lysyl substrate. Ser-107 is positioned to donate a hydrogen bond to the leaving group nitrogen. Consistent with a role in acid-base catalysis, the Hint S107A mutant protein displayed depressed catalytic activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.