Evidence map›Paper›PMID 14982789›Full record

ArticleAntimicrobial agents and chemotherapy2004

Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum.

Paktiya Teja-Isavadharm, James O Peggins, Thomas G Brewer, Nicholas J White, H Kyle Webster, Dennis E Kyle

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 27 citations in OpenAlex.

  1. Pharmacokinetics andAntimicrobial agents and chemotherapy · 2020
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  17. Artemisinins.Postgraduate medical journal · 2005
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 3 countries.

Paktiya Teja-IsavadharmArmed Forces Research Institute of Medical Sciences. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. paktiya@afrims.org
James O Peggins
Thomas G Brewer
Nicholas J White
H Kyle Webster
Dennis E Kyle
Walter Reed Army Institute of Research · USArmed Forces Research Institute of Medical Science · THMahidol Oxford Tropical Medicine Research Unit · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Artemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily available and reproducible assays to monitor drug levels closely in patients. A sensitive and reproducible bioassay for the measurement of the concentrations of artemisinin derivatives in plasma and serum is described. By modifying the in vitro drug susceptibility test, it was found that antimalarial activity in plasma or serum containing an unknown concentration of drug could be equated to the known concentrations of dihydroartemisinin (DHA) required to inhibit parasite growth. Dose-response curves for a Plasmodium falciparum clone (clone W2) and DHA were used as a standard for each assay. Assays with plasma or serum spiked with DHA proved to be reproducible (coefficient of variation, <or=10.9%), with a lower limit of quantitation equivalent to 2.5 ng of DHA per ml. For plasma spiked with artesunate or artemether, there was good agreement of the results obtained by the bioassay and the concentrations measured by high-performance liquid chromatography (HPLC) with electrochemical detection. The bioassay for measurement of the antimalarial activities of artemisinin derivatives in body fluids requires a smaller volume of plasma or serum and is more sensitive than the presently available HPLC methods, can provide pharmacodynamic parameters for determination of activity against the parasite, and should enhance the design of more appropriate dosage regimens for artemisinin drugs.

Indexed as

AnimalsAntimalarialsArtemisininsArtesunateBiological AssayBiotransformationChromatography, High Pressure LiquidDose-Response Relationship, DrugElectrochemistryHot TemperatureHumansIn Vitro TechniquesMacaca mulattaMalaria, FalciparumPlasmodium falciparumReproducibility of ResultsAntimalarialsartemisininArtemisininsartenimolArtesunateSesquiterpenes

Identifiers

PMID14982789
PMCPMC353064
OpenAlexW2050762529

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.