ArticleAntimicrobial agents and chemotherapy2004
Isolation and characterization of integron-containing bacteria without antibiotic selection.
Article in Antimicrobial agents and chemotherapy, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
41 citing papers in PubMed, 162 citations in OpenAlex.
- First Isolation, Identification, and Whole-Genome Sequencing of a Multidrug-Resistant Bovine-DerivedPathogens (Basel, Switzerland) · 2026Article
- Genomic surveillance of vancomycin-resistant Enterococcus faecium: a study on Resistome, Plasmidome, and mobilome profiling.Current genetics · 2025Article
- Antimicrobial resistance in E. Coli of animal origin and discovery of a novel ICE mobile element in Northeast China.BMC veterinary research · 2023Article
- Bacterial Communities Associated with Houseflies (Microorganisms · 2023Article
- Detection of virulence, antimicrobial resistance, and heavy metal resistance properties in Vibrio anguillarum isolated from mullet (Mugil cephalus) cultured in Korea.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Comparative genome analysis of the genusFrontiers in microbiology · 2023Article
- Discovery of Highly Trimethoprim-Resistant DfrB Dihydrofolate Reductases in Diverse Environmental Settings Suggests an Evolutionary Advantage Unrelated to Antibiotic Resistance.Antibiotics (Basel, Switzerland) · 2022Article
- Prevalence and characterisation of antimicrobial resistance genes and class 1 and 2 integrons in multiresistant Escherichia coli isolated from poultry production.Scientific reports · 2022Article
- Assessing Class 1 Integron Presence in Poultry Litter Amended with Wood Biochar and Wood Vinegar.Current microbiology · 2021Article
- Mapping foodborne pathogen contamination throughout the conventional and alternative poultry supply chains.Poultry science · 2021Review
- High Prevalence and Factors Associated With the Distribution of the IntegronFrontiers in veterinary science · 2021Article
- Article
- Genomic Analysis of two NDM-1 Providencia stuartii Strains Recovered from a Single Patient.Current microbiology · 2020Article
- Detection of class 1 integron-associated gene cassettes and tetracycline resistance genes inAnnals of medicine and surgery (2012) · 2020Article
- Integron-Associated DfrB4, a Previously Uncharacterized Member of the Trimethoprim-Resistant Dihydrofolate Reductase B Family, Is a Clinically Identified Emergent Source of Antibiotic Resistance.Antimicrobial agents and chemotherapy · 2017Article
- Integron Digestive Carriage in Human and Cattle: A "One Health" Cultivation-Independent Approach.Frontiers in microbiology · 2017Article
- Diversity of gene cassettes and the abundance of the class 1 integron-integrase gene in sediment polluted by metals.Extremophiles : life under extreme conditions · 2016Article
- Antimicrobial resistance and integron gene cassette arrays in commensal Escherichia coli from human and animal sources in IRI.Gut pathogens · 2016Article
- Safety Evaluation of Neo Transgenic Pigs by Studying Changes in Gut Microbiota Using High-Throughput Sequencing Technology.PloS one · 2016Article
- Detection of antibiotic resistance, virulence gene determinants and biofilm formation in Aeromonas species isolated from cattle.Environmental science and pollution research international · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The emergence of antibiotic resistance among pathogenic and commensal bacteria has become a serious problem worldwide. The use and overuse of antibiotics in a number of settings are contributing to the development of antibiotic-resistant microorganisms. The class 1 and 2 integrase genes (intI1 and intI2, respectively) were identified in mixed bacterial cultures enriched from bovine feces by growth in buffered peptone water (BPW) followed by integrase-specific PCR. Integrase-positive bacterial colonies from the enrichment cultures were then isolated by using hydrophobic grid membrane filters and integrase-specific gene probes. Bacterial clones isolated by this technique were then confirmed to carry integrons by further testing by PCR and DNA sequencing. Integron-associated antibiotic resistance genes were detected in bacteria such as Escherichia coli, Aeromonas spp., Proteus spp., Morganella morganii, Shewanella spp., and urea-positive Providencia stuartii isolates from bovine fecal samples without the use of selective enrichment media containing antibiotics. Streptomycin and trimethoprim resistance were commonly associated with integrons. The advantages conferred by this methodology are that a wide variety of integron-containing bacteria may be simultaneously cultured in BPW enrichments and culture biases due to antibiotic selection can be avoided. Rapid and efficient identification, isolation, and characterization of antibiotic resistance-associated integrons are possible by this protocol. These methods will facilitate greater understanding of the factors that contribute to the presence and transfer of integron-associated antibiotic resistance genes in bacterial isolates from red meat production animals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.