ArticleProceedings of the National Academy of Sciences of the United States of America2004
Molecular chaperones function as steroid receptor nuclear mobility factors.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 143 citations in OpenAlex.
- Hsp70: A Multifunctional Chaperone in Maintaining Proteostasis and Its Implications in Human Disease.Cells · 2025Review
- Stress Molecular Signaling in Interaction With Cognition.Biological psychiatry · 2025Review
- Treating the Side Effects of Exogenous Glucocorticoids; Can We Separate the Good From the Bad?Endocrine reviews · 2023Review
- Heat-Stress Preconditioning Attenuates Behavioral Responses to Psychological Stress: The Role of HSP-70 in Modulating Stress Responses.International journal of molecular sciences · 2022Article
- The Role of Histone Deacetylase 3 Complex in Nuclear Hormone Receptor Action.International journal of molecular sciences · 2021Review
- Genome-wide off-rates reveal how DNA binding dynamics shape transcription factor function.Molecular systems biology · 2020Article
- The FKBP51 Glucocorticoid Receptor Co-Chaperone: Regulation, Function, and Implications in Health and Disease.International journal of molecular sciences · 2017Review
- Negative Correlation between the Diffusion Coefficient and Transcriptional Activity of the Glucocorticoid Receptor.International journal of molecular sciences · 2017Article
- Divergent Binding and Transactivation by Two Related Steroid Receptors at the Same Response Element.The Journal of biological chemistry · 2016Article
- Moving Beyond the Androgen Receptor (AR): Targeting AR-Interacting Proteins to Treat Prostate Cancer.Hormones & cancer · 2016Review
- Ubiquitylation of nuclear receptors: new linkages and therapeutic implications.Journal of molecular endocrinology · 2015Review
- Enhancement of stress resilience through histone deacetylase 6-mediated regulation of glucocorticoid receptor chaperone dynamics.Biological psychiatry · 2015Article
- NF-κB transcriptional activity is modulated by FK506-binding proteins FKBP51 and FKBP52: a role for peptidyl-prolyl isomerase activity.The Journal of biological chemistry · 2014Article
- Systematic nucleo-cytoplasmic trafficking of proteins following exposure of MCF7 breast cancer cells to estradiol.Journal of proteome research · 2014Article
- Control of steroid receptor dynamics and function by genomic actions of the cochaperones p23 and Bag-1L.Nuclear receptor signaling · 2014Review
- Modulation of androgen receptor by FOXA1 and FOXO1 factors in prostate cancer.International journal of biological sciences · 2014Review
- Quantitation of glucocorticoid receptor DNA-binding dynamics by single-molecule microscopy and FRAP.PloS one · 2014Article
- Complex genomic interactions in the dynamic regulation of transcription by the glucocorticoid receptor.Molecular and cellular endocrinology · 2013Review
- Eukaryotic transcriptional dynamics: from single molecules to cell populations.Nature reviews. Genetics · 2013Review
- Gedunin inactivates the co-chaperone p23 protein causing cancer cell death by apoptosis.The Journal of biological chemistry · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Live cell imaging has revealed the rapid mobility of steroid hormone receptors within nuclei and their dynamic exchange at transcriptionally active target sites. Although a number of other proteins have been shown to be highly mobile within nuclei, the identity of soluble factors responsible for orchestrating nuclear trafficking remains unknown. We have developed a previously undescribed in situ subnuclear trafficking assay that generates transcriptionally active nuclei, which are depleted of soluble factors required for the nuclear mobility of glucocorticoid (GR) and progesterone receptors (PR). Using this system and a fluorescence recovery after photobleaching technique, we demonstrate that nuclear mobility of GR recovered on incubation with reticulocyte lysate was inhibited by geldanamycin, a drug that blocks the chaperone activity of heat-shock protein 90. Direct proof of molecular chaperone involvement in steroid receptor subnuclear trafficking was provided by the ATP-dependent recovery of nuclear mobility of GR and PR on incubation with various combinations of purified chaperone and/or cochaperone proteins. Additionally, for both receptors, the inclusion of hormone during the recovery period leads to a retardation of nuclear mobility. Thus, our results provide a description of soluble nuclear mobility factors and furthermore demonstrate a previously unrecognized role for molecular chaperones in the regulation of steroid receptor function within the nucleus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.