ArticleMolecular biology of the cell2004
A mechanistic model for the organization of microtubule asters by motor and non-motor proteins in a mammalian mitotic extract.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
- Physical determinants of bipolar mitotic spindle assembly and stability in fission yeast.Science advances · 2017Article
- Mitosis.Cold Spring Harbor perspectives in biology · 2016Review
- Self-organization of stabilized microtubules by both spindle and midzone mechanisms in Xenopus egg cytosol.Molecular biology of the cell · 2013Article
- Biophysics of mitosis.Quarterly reviews of biophysics · 2012Review
- Article
- Mitotic membrane helps to focus and stabilize the mitotic spindle.Biophysical journal · 2010Article
- Dynein antagonizes eg5 by crosslinking and sliding antiparallel microtubules.Current biology : CB · 2009Article
- Murine CENP-F regulates centrosomal microtubule nucleation and interacts with Hook2 at the centrosome.Molecular biology of the cell · 2009Article
- SADB phosphorylation of gamma-tubulin regulates centrosome duplication.Nature cell biology · 2009Article
- Kinesin-14 family proteins HSET/XCTK2 control spindle length by cross-linking and sliding microtubules.Molecular biology of the cell · 2009Article
- Spindle fusion requires dynein-mediated sliding of oppositely oriented microtubules.Current biology : CB · 2009Article
- The elasticity of motor-microtubule bundles and shape of the mitotic spindle.Physical biology · 2009Article
- Spatial regulation improves antiparallel microtubule overlap during mitotic spindle assembly.Biophysical journal · 2008Article
- Structural and regulatory roles of nonmotor spindle proteins.Current opinion in cell biology · 2008Review
- Getting in sync with dimeric Eg5. Initiation and regulation of the processive run.The Journal of biological chemistry · 2008Article
- Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.Molecular biology of the cell · 2007Article
- Dimeric Eg5 maintains processivity through alternating-site catalysis with rate-limiting ATP hydrolysis.The Journal of biological chemistry · 2006Article
- Eg5 steps it up!Cell division · 2006Article
- Pathway of ATP hydrolysis by monomeric kinesin Eg5.Biochemistry · 2006Article
- Kinesin-5 motors are required for organization of spindle microtubules in Silvetia compressa zygotes.BMC plant biology · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We used computer simulation to understand the functional relationships between motor (dynein, HSET, and Eg5) and non-motor (NuMA) proteins involved in microtubule aster organization. The simulation accurately predicted microtubule organization under all combinations of motor and non-motor proteins, provided that microtubule cross-links at minus-ends were dynamic, and dynein and HSET were restricted to cross-linking microtubules in parallel orientation only. A mechanistic model was derived from these data in which a combination of two aggregate properties, Net Minus-end-directed Force and microtubule Cross-linking Orientation Bias, determine microtubule organization. This model uses motor and non-motor proteins, accounts for motor antagonism, and predicts that alterations in microtubule Cross-linking Orientation Bias should compensate for imbalances in motor force during microtubule aster formation. We tested this prediction in the mammalian mitotic extract and, consistent with the model, found that increasing the contribution of microtubule cross-linking by NuMA compensated for the loss of Eg5 motor activity. Thus, this model proposes a precise mechanism of action of each noncentrosomal protein during microtubule aster organization and suggests that microtubule organization in spindles involves both motile forces from motors and static forces from non-motor cross-linking proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.