ArticleGenes & development2004
The promoter of the Chinese hamster ovary dihydrofolate reductase gene regulates the activity of the local origin and helps define its boundaries.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- The double life of mammalian DNA replication origins.Genes & development · 2025Review
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- Differences in firing efficiency, chromatin, and transcription underlie the developmental plasticity of theGenome research · 2019Article
- Dynamics of DNA replication in a eukaryotic cell.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Eukaryotic replication origins: Strength in flexibility.Nucleus (Austin, Tex.) · 2016Review
- Post-licensing Specification of Eukaryotic Replication Origins by Facilitated Mcm2-7 Sliding along DNA.Molecular cell · 2015Article
- Peaks cloaked in the mist: the landscape of mammalian replication origins.The Journal of cell biology · 2015Review
- The hunt for origins of DNA replication in multicellular eukaryotes.F1000prime reports · 2015Review
- The chromatin backdrop of DNA replication: lessons from genetics and genome-scale analyses.Biochimica et biophysica acta · 2012Review
- USF binding sequences from the HS4 insulator element impose early replication timing on a vertebrate replicator.PLoS biology · 2012Article
- Genome-wide depletion of replication initiation events in highly transcribed regions.Genome research · 2011Article
- The effect of the intra-S-phase checkpoint on origins of replication in human cells.Genes & development · 2011Article
- Eukaryotic DNA replication origins: many choices for appropriate answers.Nature reviews. Molecular cell biology · 2010Review
- Arabidopsis thaliana chromosome 4 replicates in two phases that correlate with chromatin state.PLoS genetics · 2010Article
- Genomic study of replication initiation in human chromosomes reveals the influence of transcription regulation and chromatin structure on origin selection.Molecular biology of the cell · 2010Article
- Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading.Genome research · 2010Article
- A winding road to origin discovery.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Transcription initiation activity sets replication origin efficiency in mammalian cells.PLoS genetics · 2009Article
- Differential targeting of Tetrahymena ORC to ribosomal DNA and non-rDNA replication origins.The EMBO journal · 2009Article
- A revisionist replicon model for higher eukaryotic genomes.Journal of cellular biochemistry · 2008Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The dihydrofolate reductase (DHFR) and 2BE2121 genes in the Chinese hamster are convergently transcribed in late G1 and ea ly S phase, and bracket an early-firing origin of replication that consists of a 55-kb zone of potential initiation sites. To test whether transcription through the DHFR gene is required to activate this origin in early S phase, we examined the two-dimension (2D) gel patterns of replication intermediates from several variants in which parts or all of the DHFR promote had been deleted. In those variants in which transcription was undetectable, initiation in the intergenic space was markedly suppressed (but not eliminated) in early S phase. Further more, replication of the locus required virtually the entire S period, as opposed to the usual 3-4 h. However, restoration of transcription with either the wild-type Chinese hamster promote or a Drosophila-based construct restored origin activity to the wild-type pattern. Surprisingly, 2D gel analysis of promote less variants revealed that initiation occurs at a low level in ea ly S phase not only in the intergenic region, but also in the body of the DHFR gene. The latter phenomenon has never been observed in the wild-type locus. These studies suggest that transcription through the gene normally increases the efficiency of origin firing in early S phase, but also suppresses initiation in the body of the gene, thus helping to define the boundaries of the downstream origin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.