ArticleThe British journal of ophthalmology2004
T lymphocyte mediated lysis of mitomycin C treated Tenon's capsule fibroblasts.
Article in The British journal of ophthalmology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- Interventions for late trabeculectomy bleb leak.The Cochrane database of systematic reviews · 2012Pooled it
- ISRCTN12125882 - Influence of topical anti-VEGF (Ranibizumab) on the outcome of filtration surgery for glaucoma - Study Protocol.BMC ophthalmology · 2011Trial
- Genetically Engineered Mesenchymal Stem Cell Therapy Against Murine Experimental Autoimmune Encephalomyelitis.Molecular neurobiology · 2022Article
- A Critical Overview of the Biological Effects of Mitomycin C Application on the Cornea Following Refractive Surgery.Advances in therapy · 2019Review
- Calibration of Multi-Parameter Models of Avascular Tumor Growth Using Time Resolved Microscopy Data.Scientific reports · 2018Article
- The Human IL-23 Decoy Receptor Inhibits T-Cells Producing IL-17 by Genetically Engineered Mesenchymal Stem Cells.International journal of cell biology · 2018Article
- In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon's fibroblasts: comparisons with mitomycin C.Journal of cellular and molecular medicine · 2012Article
- Scavenger receptor class B, type I (SR-BI) homo-dimerizes via its C-terminal region: fluorescence resonance energy transfer analysis.Biochimica et biophysica acta · 2007Article
- Trypan blue identifies antimetabolite treatment area in trabeculectomy.The British journal of ophthalmology · 2005Article
- Adenoviral p53 gene transfer inhibits human Tenon's capsule fibroblast proliferation.The British journal of ophthalmology · 2005Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo evaluate the effect of T cell co-culture on mitomycin C treated and untreated Tenon's capsule fibroblasts.
methodsIL-2 dependent allogeneic T cells were incubated over a monolayer of mitomycin C treated or control fibroblasts. Fibroblast numbers were evaluated by direct counts using phase contrast microscopy. To determine whether T cell mediated lysis was a consequence of MHC mismatch, co-culture experiments were repeated with autologous T cells. The effect of Fas receptor blockade was established by co-incubation with a Fas blocking (M3) antibody.
resultsT cell co-culture resulted in a dramatic reduction in fibroblast survival compared to mitomycin C treatment alone (p = 0.032). T cell killing required fibroblast/lymphocyte cell to cell contact and was observed in both allogeneic and autologous co-culture experiments. Fas blocking antibodies did not significantly inhibit T cell killing (p = 0.39).
conclusionT cells augment mitomycin C treated fibroblast death in vitro. Similar mechanisms may contribute to the cytotoxic effect of mitomycin C in vivo and account for the largely hypocellular drainage blebs that are observed clinically.
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