ArticleThe British journal of ophthalmology2004
Human preocular mucins reflect changes in surface physiology.
Article in The British journal of ophthalmology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The trial behind it
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Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- [Analysis of ocular surface alterations following proton beam radiation in eyes with conjunctival malignant melanoma].Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft · 2006Trial
- Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.Investigative ophthalmology & visual science · 2024Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Mass spectrometry analysis of human tear fluid biomarkers specific for ocular and systemic diseases in the context of 3P medicine.The EPMA journal · 2021Review
- Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.International journal of molecular sciences · 2021Article
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- Engineering drug delivery systems to overcome mucosal barriers for immunotherapy and vaccination.Tissue barriers · 2020Article
- Review of Biomarkers in Ocular Matrices: Challenges and Opportunities.Pharmaceutical research · 2019Review
- IL-13 Stimulates Proliferation and Expression of Mucin and Immunomodulatory Genes in Cultured Conjunctival Goblet Cells.Investigative ophthalmology & visual science · 2015Article
- Glycobiology of the ocular surface: mucins and lectins.Japanese journal of ophthalmology · 2013Review
- Fibroblast growth factor 10 upregulates the expression of mucins in rat conjunctival epithelial cells.Molecular vision · 2011Article
- Evaluation of ocular surface glycocalyx using lectin-conjugated fluorescein.Clinical ophthalmology (Auckland, N.Z.) · 2010Article
- Functions of ocular surface mucins in health and disease.Current opinion in allergy and clinical immunology · 2008Review
- Mucins in the mucosal barrier to infection.Mucosal immunology · 2008Review
- MUC16 expression in Sjogren's syndrome, KCS, and control subjects.Molecular vision · 2008Article
- Sequence-based estimation of minisatellite and microsatellite repeat variability.Genome research · 2007Article
- MUC5AC overexpression in tear film of neonates.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2007Article
- Assay of mucins in human tear fluid.Experimental eye research · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
BACKGROUND/
aimsMucin function is associated with both peptide core and glycosylation characteristics. The authors assessed whether structural alterations occurring during mucin residence in the tear film reflect changes in ocular surface physiology.
methodsOcular surface mucus was collected from normal volunteers as N-acetyl cysteine (NAcCys) washes or directly from the speculum after cataract surgery. To assess the influence of surface health on mucins, NAcCys washings were also obtained from patients with symptoms, but no clinical signs of dry eye (symptomatics). Mucins were extracted in guanidine hydrochloride (GuHCl) with protease inhibitors. Buoyant density of mucin species, a correlate of glycosylation density, was followed by reactivity with anti-peptide core antibodies. Mucin hydrodynamic volume was assessed by gel filtration on Sepharose CL2B.
resultsSurface fluid and mucus contained soluble forms of MUC1, MUC2, MUC4, and MUC5AC and also the same species requiring DTT solubilisation. Reactivity with antibodies to MUC2 and MUC5AC peaked at 1.3-1.5 g/ml in normals, while dominated by underglycosylated forms in symptomatics. Surface mucins were predominantly smaller than intracellular species. MUC2 size distributions were different in symptomatics and normals, while those of MUC5AC were similar in these two groups.
conclusionsA reduction in surface mucin size indicates post-secretory cleavage. Dissimilarities in surface mucin glycosylation and individual MUC size distributions in symptomatics suggest changes in preocular mucin that might precede dry eye signs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.