ArticleMolecular and cellular biochemistry
Studies of mitochondrial respiration in muscle cells in situ: use and misuse of experimental evidence in mathematical modelling.
Article in Molecular and cellular biochemistry. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 20 citations in OpenAlex.
- Role of DJ-1 in the mechanism of pathogenesis of Parkinson's disease.Journal of bioenergetics and biomembranes · 2019Review
- Exercise myopathy: changes in myofibrils of fast-twitch muscle fibres.Biology of sport · 2014Article
- Structure and functional characteristics of rat's left ventricle cardiomyocytes under antiorthostatic suspension of various duration and subsequent reloading.Journal of biomedicine & biotechnology · 2012Article
- Inhibiting myosin-ATPase reveals a dynamic range of mitochondrial respiratory control in skeletal muscle.The Biochemical journal · 2011Article
- Study of possible interactions of tubulin, microtubular network, and STOP protein with mitochondria in muscle cells.Molecular and cellular biochemistry · 2010Article
- Intracellular diffusion restrictions in isolated cardiomyocytes from rainbow trout.BMC cell biology · 2009Article
- Philosophical basis and some historical aspects of systems biology: from Hegel to Noble - applications for bioenergetic research.International journal of molecular sciences · 2009Review
- Mitochondrial OXPHOS functions in R1H rhabdomyosarcoma and skeletal muscles of the rat.Neurochemical research · 2007Article
- Compartmentation of energy metabolism in atrial myocardium of patients undergoing cardiac surgery.Molecular and cellular biochemistry · 2005Article
- Intracellular energetic units in healthy and diseased hearts.Experimental and clinical cardiology · 2005Article
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Applications of permeabilized cell and skinned fiber techniques in combination with methods of mathematical modelling for studies of mitochondrial function in the cell are critically evaluated. Mathematical models may be useful tools for explaining biological phenomena, but only if they are selected by fitting the computing results with real experimental data. Confocal microscopy has been used in experiments with permeabilized cardiomyocytes and myocardial fibers to determine the maximal diffusion distance from medium to the core of cells, which is shown not to exceed 8-10 microm. This is a principal index for correctly explaining high apparent Km for exogenous ADP (200-300 microM) in regulation of mitochondrial respiration in oxidative muscle cells in situ. The best fitting of the results of in silico studies may be achieved by using of the compartmentalized energy transfer model. From these results, it may be concluded that in cardiac muscle cells the mitochondria and ATPases are organized into intracellular energetic units (ICEUs) separated from the bulk phase of cytoplasm by some barriers which limit the diffusion of adenine nucleotides. In contrast, alternative models based on the concept of the cell as homogenous system do not explain the observed experimental phenomena and have led to misleading conclusions. The various sources of experimental and conceptual errors are analyzed.
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