ArticleVirus genes2004
The application of alkaline phosphatase labeled HBV probe in serum detection.
Article in Virus genes, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed, 3 citations in OpenAlex.
- Characterization of alkaline phosphatase labeled UidA(Gus) probe and its application in testing of transgenic tritordeum.Molecular biology reports · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A method using non-radioactive material alkaline phosphatase to label HBV DNA as probe has been studied and used in clinical experiments to detect the HBV DNA in hepatitis serum. Alkaline phosphatase coupled with polyethyleneimine (PEI) using P-benzoquine as cross-linking reagent. The modified phosphatase was covalently linked to single strand DNA using glutraldehyde. Such single strand DNA enzyme complexes have been tested for blot hybridization, after hybridization and incubation with a substrate solution, sequences complementary to the probe can be visualized directly in 1 h. The minimum amount about 10 pg of target DNA has been detected in this way, 32P labeled probes are autoradiography 1 h after hybridization can only detect 10 ng, so the enzyme labeled probe is more sensitive than isotope labeled probe in 1 h fast test. Comparing the enzyme-labeled HBV DNA probe with 32P labeled the same one, positive proportion of detecting the HBV DNA in hepatitis patients was about 95.7%. Because the positive patient's serum detected by 32P labeled probe were selected through 1- week radiation, Alkaline Phosphatase labeled probes are color developed for only 1 h. Our experiment certified that it is a sensitive, specific, easy, rapid, safe and economical probe labeling and clinical virus DNA detection method.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.