ArticlePlant physiology2004
Manipulation of endogenous trypsin proteinase inhibitor production in Nicotiana attenuata demonstrates their function as antiherbivore defenses.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 248 citations in OpenAlex.
- MYC2a/b-regulated protease inhibitors in defense against Spodoptera litura in Nicotiana attenuata.Plant cell reports · 2025Article
- The emergence and loss of cyclic peptides inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- NaMLP, a new identified Kunitz trypsin inhibitor regulated synergistically by JA and ethylene, confers Spodoptera litura resistance in Nicotiana attenuata.Plant cell reports · 2023Article
- Multi-locus genome-wide association study of chickpea reference set identifies genetic determinants ofFrontiers in plant science · 2023Article
- The downside of metabolic diversity: Postingestive rearrangements by specialized insects.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Defensive capabilities of contrasting sorghum genotypes against Atherigona soccata (Rondani) infestation.Protoplasma · 2022Article
- Proteinase inhibitors in legume herbivore defense: from natural to genetically engineered protectants.Plant cell reports · 2022Review
- TcTI, a Kunitz-type trypsin inhibitor from cocoa associated with defense against pathogens.Scientific reports · 2022Article
- Water Deficiency and Induced Defense Against a Generalist Insect Herbivore in Desert and Mediterranean Populations of Eruca sativa.Journal of chemical ecology · 2021Article
- Characterization of a Bowman-Birk type trypsin inhibitor purified from seeds of Solanum surattense.Scientific reports · 2021Article
- NaKTI2, a Kunitz trypsin inhibitor transcriptionally regulated by NaWRKY3 and NaWRKY6, is required for herbivore resistance in Nicotiana attenuata.Plant cell reports · 2021Article
- Strigolactone signaling regulates specialized metabolism in tobacco stems and interactions with stem-feeding herbivores.PLoS biology · 2020Article
- Oral cues are not enough: induction of defensive proteins in Nicotiana tabacum upon feeding by caterpillars.Planta · 2020Article
- Genome-Wide Identification and Expression Analysis of the Protease Inhibitor Gene Families in Tomato.Genes · 2019Article
- Phytohormones in Fall Armyworm Saliva Modulate Defense Responses in Plants.Journal of chemical ecology · 2019Article
- Can Plant Defence Mechanisms Provide New Approaches for the Sustainable Control of the Two-Spotted Spider Mite Tetranychus urticae?International journal of molecular sciences · 2018Review
- Flower-specific jasmonate signaling regulates constitutive floral defenses in wild tobacco.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Changes in cytokinins are sufficient to alter developmental patterns of defense metabolites in Nicotiana attenuata.The Plant journal : for cell and molecular biology · 2017Article
- Article
- Jasmonic acid protects etiolated seedlings of Arabidopsis thaliana against herbivorous arthropods.Plant signaling & behavior · 2016Article
32 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Evidence for the in planta defensive function of trypsin protease inhibitors (TPIs) comes from observations of enhanced herbivore resistance after heterologous TPI expression or the manipulation of signal cascades that activate numerous defense responses, including TPI production; no studies have altered the expression of an endogenous pi gene to examine defensive function. We isolated two genes with seven- and six-repeat TPI domains from Nicotiana attenuata from the potato (Solanum tuberosum) PI-II family. To determine whether endogenous TPIs in N. attenuata function defensively against the native herbivores, hornworm (Manduca sexta) and mirids (Tupiocoris notatus), we expressed 175 bp of the seven-domain pi from N. attenuata in an antisense orientation in a TPI-producing genotype to reduce TPI expression and expressed the full-length seven-domain pi in a sense orientation under control of a constitutive promoter to restore TPI activity in a natural genotype from Arizona unable to produce TPIs. Constitutive and inducible TPI production in two antisense lines were diminished by 80% to 90% and 33% to 52%, respectively, and sense expression restored 67% of the activity found in the TPI-producing genotype after caterpillar attack in the TPI-deficient A genotype. Hornworm larvae fed on genotypes with low or no TPI activity grew faster, had higher survivorship, and produced heavier pupae than those that fed on genotypes with high TPI activity. T. notatus showed higher preference for genotypes with low or no TPI activity than for genotypes with high TPI levels. We conclude that endogenous TPIs are an effective defense against these native herbivores.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.