ArticleThe Journal of biological chemistry2004
Activation and repression of interleukin-12 p40 transcription by erythroid Kruppel-like factor in macrophages.
Article in The Journal of biological chemistry, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 62 citations in OpenAlex.
- CCAAT/enhancer-binding protein delta regulates miRs-4257 and 3156 to attenuate the interleukin 12 through small extracellular vesicle transmission in glioblastoma.Cancer cell international · 2026Article
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- Structure-primed embedding on the transcription factor manifold enables transparent model architectures for gene regulatory network and latent activity inference.Genome biology · 2024Article
- Erythroid Krüppel-Like Factor (KLF1): A Surprisingly Versatile Regulator of Erythroid Differentiation.Advances in experimental medicine and biology · 2024Review
- Monocyte Differentiation and Heterogeneity: Inter-Subset and Interindividual Differences.International journal of molecular sciences · 2023Review
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- Evaluation of an ImmunoPET Tracer for IL-12 in a Preclinical Model of Inflammatory Immune Responses.Frontiers in immunology · 2022Article
- Negative Regulation of the Differentiation of Flk2International journal of molecular sciences · 2020Article
- MicroRNA-146a regulates the transformation from liver fibrosis to cirrhosis in patients with hepatitis B via interleukin-6.Experimental and therapeutic medicine · 2019Article
- Critical role of Jumonji domain of JMJD1C in MLL-rearranged leukemia.Blood advances · 2019Article
- Decreased expression of microRNA-30b promotes the development of pulpitis by upregulating the expression of interleukin-6 receptor.Experimental and therapeutic medicine · 2019Article
- Regulatory role of miRNA-26a in neonatal sepsis.Experimental and therapeutic medicine · 2018Article
- MicroRNA-365 regulates the occurrence and immune response of sepsis following multiple trauma via interleukin-6.Experimental and therapeutic medicine · 2018Article
- The regulatory role of IL-6R in hepatitis B-associated fibrosis and cirrhosis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- PHF6 regulates phenotypic plasticity through chromatin organization within lineage-specific genes.Genes & development · 2017Article
- Aging and the Krüppel-like factors.Trends in cell & molecular biology · 2017Article
- MicroRNA-365 in macrophages regulates Mycobacterium tuberculosis-induced active pulmonary tuberculosis via interleukin-6.International journal of clinical and experimental medicine · 2015Article
- Extrinsic and intrinsic control by EKLF (KLF1) within a specialized erythroid niche.Development (Cambridge, England) · 2014Article
- Regulation of an inflammatory disease: Krüppel-like factors and atherosclerosis.Arteriosclerosis, thrombosis, and vascular biology · 2014Review
- Klf10 inhibits IL-12p40 production in macrophage colony-stimulating factor-induced mouse bone marrow-derived macrophages.European journal of immunology · 2013Article
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 2 countries.
Funding
Abstract
Transcription of interleukin (IL)-12 p40 in myeloid cells is attributed to the recruitment of multiple activated transcription factors such as nuclear factor kappaB (NFkappaB), CCAAT enhancer-binding protein beta, ets-2, PU.1, and so forth. We now provide the first description of the human erythroid Kruppel-like factor (EKLF) in human primary macrophages and identify the role of EKLF in IL-12 p40 expression. EKLF-specific binding to the CACCC element (-224 to -220) on the human IL-12 p40 promoter was observed in resting human primary macrophages. Functional analysis of the CACCC element revealed a dependent role for EKLF binding in activating IL-12 p40 transcription in resting RAW264.7 cells, whereas EKLF overexpression in the presence or absence of this element repressed IL-12 p40 transcription in interferon gamma/lipopolysaccharide-stimulated RAW264.7 cells. Murine endogenous IL-12 p40 mRNA was consistently induced by overexpressed EKLF in resting RAW264.7 cells, whereas EKLF suppressed IL-12 p40 expression in activated RAW264.7 cells. Modulation of nuclear binding activities at the IL-12 p40 NFkappaB half-site was induced by EKLF for down-regulation of IL-12 p40 transcription in activated RAW264.7 cells, but no effect of EKLF on NFkappaB activity was observed in resting RAW264.7 cells. Taken together, we identify EKLF as a transcription factor in macrophages able to regulate IL-12 p40 transcription depending on the cellular activation status. The bifunctional control of IL-12 p40 by EKLF and its modulation of NFkappaB support a potential function for this factor in orchestrating IL-12 p40 production in macrophages.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.