ArticleNucleic acids research2004
Generating in vitro transcripts with homogenous 3' ends using trans-acting antigenomic delta ribozyme.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 21 citations in OpenAlex.
- Optimized R2 retroelement complexes for DNA insertion into plant genomes.Nature biotechnology · 2026Article
- A robust and versatile method for production and purification of large-scale RNA samples for structural biology.RNA (New York, N.Y.) · 2020Article
- Reverse Genetics for Peste des Petits Ruminants Virus: Current Status and Lessons to Learn from Other Non-segmented Negative-Sense RNA Viruses.Virologica Sinica · 2018Review
- AnIranian journal of microbiology · 2017Article
- Synthesizing topological structures containing RNA.Nature communications · 2017Article
- Syn5 RNA polymerase synthesizes precise run-off RNA products.Nucleic acids research · 2014Article
- RNA synthesis and purification for structural studies.RNA biology · 2014Review
- An RNA toolbox for single-molecule force spectroscopy studies.Nucleic acids research · 2007Article
- Antigenomic delta ribozyme variants with mutations in the catalytic core obtained by the in vitro selection method.Nucleic acids research · 2006Article
- A novel 4-base-recognizing RNA cutter that can remove the single 3' terminal nucleotides from RNA molecules.Nucleic acids research · 2004Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most in vitro run-off transcription reactions with T7 RNA polymerase, transcripts with heterogeneous ends are commonly obtained. Towards the goal of finding a simple and effective procedure for correct processing of their 3' ends we propose the use of trans-acting antigenomic delta ribozyme. We demonstrate that the extension of nascent transcripts with only seven nucleotides complementary to the ribozyme's recognition site, and subsequently, the removal of those nucleotides with the ribozyme acting in trans, is an efficient procedure for generating transcripts with homogenous 3' ends. This approach was tested on two model RNA molecules: an in vitro transcript of yeast tRNA(Phe) and a delta ribozyme, which processed itself during transcription. The proposed procedure is a simple alternative to the use of ribozymes as cis-cleaving autocatalytic cassettes attached to transcript 3' ends. As there is little possibility that the required additional stretch, only seven nucleotides long, enters into stable interactions with other parts of the transcripts, it can be cleaved off with high efficacy.
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