ArticleNucleic acids research2004
Surfection: a new platform for transfected cell arrays.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway.Cell communication and signaling : CCS · 2020Article
- Area-specific cell stimulation via surface-mediated gene transfer using apatite-based composite layers.International journal of molecular sciences · 2015Article
- Addition of ascorbic acid to the extracellular environment activates lipoplexes of a ferrocenyl lipid and promotes cell transfection.Journal of controlled release : official journal of the Controlled Release Society · 2012Article
- Article
- Data ontology and an information system realization for web-based management of image measurements.Frontiers in neuroinformatics · 2011Article
- Efficacy of immobilized polyplexes and lipoplexes for substrate-mediated gene delivery.Biotechnology and bioengineering · 2009Article
- Functional studies on transfected cell microarray analysed by linear regression modelling.Nucleic acids research · 2008Article
- Site-specific gene transfer with high efficiency onto a carbon nanotube-loaded electrode.Journal of the Royal Society, Interface · 2008Article
- Bioluminescence imaging for assessment and normalization in transfected cell arrays.Biotechnology and bioengineering · 2007Article
- Gene Delivery by Immobilization to Cell-Adhesive Substrates.MRS bulletin · 2005Article
- Substrate-mediated delivery from self-assembled monolayers: effect of surface ionization, hydrophilicity, and patterning.Acta biomaterialia · 2005Article
- High-throughput RNAi screening in vitro: from cell lines to primary cells.RNA (New York, N.Y.) · 2005Article
- Spatially and temporally controlled gene transfer by electroporation into adherent cells on plasmid DNA-loaded electrodes.Nucleic acids research · 2004Article
- High-throughput gene silencing using cell arrays.Oncogene · 2004Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Efficient high-throughput expression of genes in mammalian cells can facilitate large-scale functional genomic studies. Towards this aim, we developed a simple yet powerful method to deliver genes into cells by cationic polymers on the surface of substrates. Transfection can be achieved by directly contacting nucleic acid-cell mixtures with the cationic substrates, e.g. polyethylenimine/collagen-coated wells. This single-step matrix-surface- mediated transfection method, termed 'surfection', can efficiently deliver multiple plasmids into cells and can successfully assay siRNA-mediated gene silencing. This technology represents the easiest method to transfer combinations of genes in large-scale arrays, and is a versatile tool for live-cell imaging and cell-based drug screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.