ArticleNucleic acids research2004
Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 76 citations in OpenAlex.
- Enhancing the reverse transcriptase function in Taq polymerase via AI-driven multiparametric rational design.Frontiers in bioengineering and biotechnology · 2024Article
- Recycling of Polymerase Chain Reaction (PCR) Kits.ACS sustainable chemistry & engineering · 2023Article
- LNA blockers for improved amplification selectivity.Scientific reports · 2023Article
- Measurable Residual Disease Monitoring by Locked Nucleic Acid Quantitative Real-Time PCR Assay forCancers · 2022Article
- Allele-Specific Recombinase Polymerase Amplification to Detect Sickle Cell Disease in Low-Resource Settings.Analytical chemistry · 2021Article
- PANDAA intentionally violates conventional qPCR design to enable durable, mismatch-agnostic detection of highly polymorphic pathogens.Communications biology · 2021Article
- A highly sensitive and selective signal-on strategy for microRNA quantification.Analytica chimica acta · 2020Article
- Wild‑type blocking pcr coupled with internal competitive amplified fragment improved the detection of rare mutation of KRAS.Molecular medicine reports · 2017Article
- Article
- Characterization of polybacterial clinical samples using a set of group-specific broad-range primers targeting the 16S rRNA gene followed by DNA sequencing and RipSeq analysis.Journal of medical microbiology · 2011Article
- Article
- Article
- Selectivity of Enzymatic Conversion of Oligonucleotide Probes during Nucleotide Polymorphism Analysis of DNA.Acta naturae · 2010Article
- Ultrasensitive genotypic detection of antiviral resistance in hepatitis B virus clinical isolates.Antimicrobial agents and chemotherapy · 2009Article
- Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.Environmental microbiology · 2008Article
- Characterization of aberrant melting peaks in unlabeled probe assays.The Journal of molecular diagnostics : JMD · 2007Article
- Analysis of a nuclease activity of catalytic domain of Thermus thermophilus MutS2 by high-accuracy mass spectrometry.Nucleic acids research · 2007Article
- Position-dependent effects of locked nucleic acid (LNA) on DNA sequencing and PCR primers.Nucleic acids research · 2006Article
- Locked nucleic acid: high-affinity targeting of complementary RNA for RNomics.Handbook of experimental pharmacology · 2006Review
- Proximity extension of circular DNA aptamers with real-time protein detection.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of locked nucleic acid (LNA) modification position upon representative DNA polymerase and exonuclease activities has been examined for potential use in primer extension genotyping applications. For the 3'-->5' exonuclease activities of four proofreading DNA polymerases (Vent, Pfu, Klenow fragment and T7 DNA polymerase) as well as exonuclease III, an LNA at the terminal (L-1) position of a primer is found to provide partial protection against the exonucleases of the two family B polymerases only. In contrast, an LNA residue at the penultimate (L-2) position generates essentially complete nuclease resistance. The polymerase active sites of these enzymes also display a distinct preference. An L-1 LNA modification has modest effects upon poly merization, but an L-2 LNA group slows dTTP incorporation somewhat while virtually abolishing extension with ddTTP or acyTTP terminators, even with A488L Vent DNA polymerase engineered for terminator incorporation. These observations on active site preference have been utilized to demonstrate two novel assays: exonuclease-mediated single base extension (E-SBE) and proofreading allele-specific extension (PRASE). We show that a model PRASE genotyping reaction with L-2 LNA primers offers greater specificity than existing non-proofreading assays, whether or not the non-proofreading reaction employs LNA-modified primers.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.