ArticleNucleic acids research2004
Overexpression of phage-type RNA polymerase RpoTp in tobacco demonstrates its role in chloroplast transcription by recognizing a distinct promoter type.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Professor Thomas Börner: the pioneer of molecular plant genetics in East Germany turns 80!Molecular genetics and genomics : MGG · 2026Article
- A Transcriptomic Analysis of Tobacco Leaf with the Functional Loss of the PlastidPlants (Basel, Switzerland) · 2022Article
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The plastid transcription machinery and its coordination with the expression of nuclear genome: Plastid-Encoded Polymerase, Nuclear-Encoded Polymerase and the Genomes Uncoupled 1-mediated retrograde communication.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- The caseinolytic protease complex component CLPC1 in Arabidopsis maintains proteome and RNA homeostasis in chloroplasts.BMC plant biology · 2018Article
- G196 epitope tag system: a novel monoclonal antibody, G196, recognizes the small, soluble peptide DLVPR with high affinity.Scientific reports · 2017Article
- Comparative Analysis of ChloroplastFrontiers in plant science · 2017Article
- Ectopic expression of an Arabidopsis dehydration-responsive element-binding factor DREB2C improves salt stress tolerance in crucifers.Plant cell reports · 2014Article
- Mitochondrial energy and redox signaling in plants.Antioxidants & redox signaling · 2013Review
- Arabidopsis CSP41 proteins form multimeric complexes that bind and stabilize distinct plastid transcripts.Journal of experimental botany · 2012Article
- Evolution of plant phage-type RNA polymerases: the genome of the basal angiosperm Nuphar advena encodes two mitochondrial and one plastid phage-type RNA polymerases.BMC evolutionary biology · 2010Article
- Phage-type RNA polymerase RPOTmp performs gene-specific transcription in mitochondria of Arabidopsis thaliana.The Plant cell · 2009Article
- Evolution of phage-type RNA polymerases in higher plants: characterization of the single phage-type RNA polymerase gene from Selaginella moellendorffii.Journal of molecular evolution · 2009Article
- Post-transcriptional control of chloroplast gene expression.Gene regulation and systems biology · 2009Article
- Impaired function of the phage-type RNA polymerase RpoTp in transcription of chloroplast genes is compensated by a second phage-type RNA polymerase.Nucleic acids research · 2008Article
- High diversity of plastidial promoters in Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2007Article
- In vivo visualization of Mg-protoporphyrin IX, a coordinator of photosynthetic gene expression in the nucleus and the chloroplast.The Plant cell · 2007Article
- Arabidopsis phage-type RNA polymerases: accurate in vitro transcription of organellar genes.The Plant cell · 2007Article
- Article
- pTAC2, -6, and -12 are components of the transcriptionally active plastid chromosome that are required for plastid gene expression.The Plant cell · 2006Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Plant cells possess three DNA-containing compartments, the nucleus, the mitochondria and the plastids. Accordingly, plastid gene regulation is fairly complex. Albeit plastids retained their own genome and prokaryotic-type gene expression system by a plastid-encoded RNA polymerase (PEP), they need a second nuclear-encoded plastid transcription activity, NEP. Candidate genes for putative NEP catalytic subunits have been cloned in Arabidopsis thaliana (AtRpoTp) and Nicotiana sylvestris (NsRpoTp). To provide evidence for RpoTp as a gene encoding a NEP catalytic subunit, we introduced the AtRpoTp and NsRpoTp cDNAs into the tobacco nucleus under the control of the strong constitutive CaMV 35S promoter. Analysis of transcription from NEP and PEP promoters in these transgenic plants using primer extension assays revealed enhanced transcription from typical type I NEP promoters as PatpB-289 in comparison with the wild type. These data provide direct evidence that RpoTp is a catalytic subunit of NEP and involved in recognition of a distinct subset of type I NEP promoters.
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