ArticleThe Plant cell2004
The F-box protein AhSLF-S2 physically interacts with S-RNases that may be inhibited by the ubiquitin/26S proteasome pathway of protein degradation during compatible pollination in Antirrhinum.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 196 citations in OpenAlex.
- The Ubiquitin-Proteasome System in Flowering Plant Reproduction: Mechanisms, Functional Diversity, and Regulatory Networks.Plants (Basel, Switzerland) · 2026Review
- S-RNase-based self-incompatibility in angiosperms: Degradation, condensation, and evolution.Plant physiology · 2025Review
- Origin of a self-compatibility associated MITE in Petota and its application in hybrid potato breeding.The New phytologist · 2025Article
- Rapid detection of RNase-based self-incompatibility in Lysimachia monelli (Primulaceae).American journal of botany · 2025Article
- Potato: from functional genomics to genetic improvement.Molecular horticulture · 2024Review
- The Identification and Analysis of the Self-Incompatibility Pollen Determinant FactorPlants (Basel, Switzerland) · 2024Article
- Epididymal RNase T2 contributes to astheno-teratozoospermia and intergenerational metabolic disorder through epididymosome-sperm interaction.BMC medicine · 2023Article
- The Pitaya Flower Tissue's Gene Differential Expression Analysis between Self-Incompatible and Self-Compatible Varieties for the Identification of Genes Involved in Self-Incompatibility Regulation.International journal of molecular sciences · 2023Article
- PbrBZR1 interacts with PbrARI2.3 to mediate brassinosteroid-regulated pollen tube growth during self-incompatibility signaling in pear.Plant physiology · 2023Article
- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- Molecular Approaches to Overcome Self-Incompatibility in Diploid Potatoes.Plants (Basel, Switzerland) · 2022Review
- Genetic control of compatibility in crosses between wheat and its wild or cultivated relatives.Plant biotechnology journal · 2022Review
- Origin, loss, and regain of self-incompatibility in angiosperms.The Plant cell · 2022Article
- Gene coexpression analysis reveals key pathways and hub genes related to late-acting self-incompatibility inFrontiers in plant science · 2022Article
- Primary restriction of S-RNase cytotoxicity by a stepwise ubiquitination and degradation pathway in Petunia hybrida.The New phytologist · 2021Article
- Trans-acting regulators of ribonuclease activity.Journal of microbiology (Seoul, Korea) · 2021Review
- The 26S Proteasome Regulatory Subunit GmPSMD Promotes Resistance toFrontiers in plant science · 2021Article
- Review
- Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology.Frontiers in plant science · 2020Review
- A gamma-thionin protein from apple, MdD1, is required for defence against S-RNase-induced inhibition of pollen tube prior to self/non-self recognition.Plant biotechnology journal · 2019Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Self-incompatibility S-locus-encoded F-box (SLF) proteins have been identified in Antirrhinum and several Prunus species. Although they appear to play an important role in self-incompatible reaction, functional evidence is lacking. Here, we provide several lines of evidence directly implicating a role of AhSLF-S(2) in self-incompatibility in Antirrhinum. First, a nonallelic physical interaction between AhSLF-S(2) and S-RNases was demonstrated by both coimmunoprecipitation and yeast two-hybrid assays. Second, AhSLF-S(2) interacts with ASK1- and CULLIN1-like proteins in Antirrhinum, and together, they likely form an Skp1/Cullin or CDC53/F-box (SCF) complex. Third, compatible pollination was specifically blocked after the treatment of the proteasomal inhibitors MG115 and MG132, but they had little effect on incompatible pollination both in vitro and in vivo, indicating that the ubiquitin/26S proteasome activity is involved in compatible pollination. Fourth, the ubiquitination level of style proteins was increased substantially after compatible pollination compared with incompatible pollination, and coimmunoprecipitation revealed that S-RNases were ubiquitinated after incubating pollen proteins with compatible but not with incompatible style proteins, suggesting that non-self S-RNases are possibly degraded by the ubiquitin/26S proteasome pathway. Fifth, the S-RNase level appeared to be reduced after 36 h of compatible pollination. Taken together, these results show that AhSLF-S(2) interacts with S-RNases likely through a proposed SCF(AhSLF-S2) complex that targets S-RNase destruction during compatible rather than incompatible pollination, thus providing a biochemical basis for the inhibition of pollen tube growth as observed in self-incompatible response in Antirrhinum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.