ArticleThe Plant cell2004
Microarray expression analyses of Arabidopsis guard cells and isolation of a recessive abscisic acid hypersensitive protein phosphatase 2C mutant.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 233 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
233 citing papers in PubMed, 552 citations in OpenAlex.
- Cell-type-specific compartmentalization and function of the glucosinolate-myrosinase system in Arabidopsis thaliana.The Journal of biological chemistry · 2026Article
- FIMBRIN2 Regulates ABA-Induced Stomatal Closure by Promoting Microfilament Bundling and Turnover in Arabidopsis Guard Cells.Plant direct · 2026Article
- The RCAR12-CAP1-OST1 module controls ABA-mediated stomatal closure in Arabidopsis.PLoS genetics · 2026Article
- OPEN STOMATA 1 activates SLAC1 anion channel primarily through CPK15 in ABA-induced stomatal closure inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- INTACT-based guard cell transcriptomes from a progressive drought time course reveal targets for modifying stomatal responses.The Plant cell · 2025Article
- The Emerging Role of the Salt Tolerance-Related Protein in the Abiotic Stress Response ofPlants (Basel, Switzerland) · 2025Review
- A Genome-Wide Association Screen for Genes Affecting Leaf Trichome Development and Epidermal Metal Accumulation in Arabidopsis.Plant, cell & environment · 2025Article
- Illuminating stomatal responses to red light: establishing the role of Ci-dependent versus -independent mechanisms in control of stomatal behaviour.Journal of experimental botany · 2024Article
- Article
- ABA INSENSITIVE 2 promotes flowering by inhibiting OST1/ABI5-dependent FLOWERING LOCUS C transcription in Arabidopsis.Journal of experimental botany · 2024Article
- Distinct guard cell-specific remodeling of chromatin accessibility during abscisic acid- and COProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Distinct guard cell specific remodeling of chromatin accessibility during abscisic acid and CObioRxiv : the preprint server for biology · 2023Article
- Differential expression of genes during recovery of Nicotiana tabacum from tomato leaf curl Gujarat virus infection.Planta · 2023Article
- PECTIN METHYLESTERASE INHIBITOR18 functions in stomatal dynamics and stomatal dimension.Plant physiology · 2023Article
- Key Pathways and Genes ofPlants (Basel, Switzerland) · 2023Article
- ASR1 and ASR2, Two Closely Related ABA-Induced Serine-Rich Transcription Repressors, Function Redundantly to Regulate ABA Responses in Arabidopsis.Plants (Basel, Switzerland) · 2023Article
- Genome-wide identification of bHLH transcription factors and their response to salt stress inFrontiers in plant science · 2023Article
- Metabolic pathways engineering for drought or/and heat tolerance in cereals.Frontiers in plant science · 2023Review
- Plant hormone signals regulate trehalose accumulation against osmotic stress in watermelon cells.Protoplasma · 2022Article
- Leaf cell-specific and single-cell transcriptional profiling reveals a role for the palisade layer in UV light protection.The Plant cell · 2022Article
173 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Oligomer-based DNA Affymetrix GeneChips representing about one-third of Arabidopsis (Arabidopsis thaliana) genes were used to profile global gene expression in a single cell type, guard cells, identifying 1309 guard cell-expressed genes. Highly pure preparations of guard cells and mesophyll cells were isolated in the presence of transcription inhibitors that prevented induction of stress-inducible genes during cell isolation procedures. Guard cell expression profiles were compared with those of mesophyll cells, resulting in identification of 64 transcripts expressed preferentially in guard cells. Many large gene families and gene duplications are known to exist in the Arabidopsis genome, giving rise to redundancies that greatly hamper conventional genetic and functional genomic analyses. The presented genomic scale analysis identifies redundant expression of specific isoforms belonging to large gene families at the single cell level, which provides a powerful tool for functional genomic characterization of the many signaling pathways that function in guard cells. Reverse transcription-PCR of 29 genes confirmed the reliability of GeneChip results. Statistical analyses of promoter regions of abscisic acid (ABA)-regulated genes reveal an overrepresented ABA responsive motif, which is the known ABA response element. Interestingly, expression profiling reveals ABA modulation of many known guard cell ABA signaling components at the transcript level. We further identified a highly ABA-induced protein phosphatase 2C transcript, AtP2C-HA, in guard cells. A T-DNA disruption mutation in AtP2C-HA confers ABA-hypersensitive regulation of stomatal closing and seed germination. The presented data provide a basis for cell type-specific genomic scale analyses of gene function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.