ArticleThe Plant cell2004
Identification of multivesicular bodies as prevacuolar compartments in Nicotiana tabacum BY-2 cells.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 192 papers, 1 of them a synthesis that pooled it.
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Who cites it
192 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Plant-Derived Nanovesicles: A Comprehensive Review from Isolation to Clinical Translation-Unlocking Natural Nanocarriers for Biomedical Applications.Biomolecules · 2026Review
- Structural Insights into Cell Wall-Related Vesicle Secretion with Different Mechanisms.Plants (Basel, Switzerland) · 2026Article
- Cargo Sorting Zones in Plant Trans-Golgi Network.Sub-cellular biochemistry · 2026Review
- RLCKs phosphorylate RopGEFs to control auxin-dependent Arabidopsis development.Nature plants · 2025Article
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- The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis.Nature communications · 2025Article
- The fate and function of theFrontiers in plant science · 2025Article
- OsSNDP4, a Sec14-nodulin Domain Protein, is Required for Pollen Development in Rice.Rice (New York, N.Y.) · 2024Article
- Homogalacturonan Pectins Tuned as an Effect of Susceptible rbohD, Col-0-Reactions, and Resistance rbohF-, rbohD/F-Reactions to TuMV.International journal of molecular sciences · 2024Article
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Localized calcium transients in phragmoplast regulate cytokinesis of tobacco BY-2 cells.Plant cell reports · 2024Article
- A CYP78As-small grain4-coat protein complex Ⅱ pathway promotes grain size in rice.The Plant cell · 2023Article
- The SYP123-VAMP727 SNARE complex delivers secondary cell wall components for root hair shank hardening in Arabidopsis.The Plant cell · 2023Article
- The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis.The Plant cell · 2023Article
- Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.Nature communications · 2023Article
132 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the dynamics and molecular components of plant prevacuolar compartments (PVCs). We have demonstrated recently that vacuolar sorting receptor (VSR) proteins are concentrated on PVCs. In this study, we generated transgenic Nicotiana tabacum (tobacco) BY-2 cell lines expressing two yellow fluorescent protein (YFP)-fusion reporters that mark PVC and Golgi organelles. Both transgenic cell lines exhibited typical punctate YFP signals corresponding to distinct PVC and Golgi organelles because the PVC reporter colocalized with VSR proteins, whereas the Golgi marker colocalized with mannosidase I in confocal immunofluorescence. Brefeldin A induced the YFP-labeled Golgi stacks but not the YFP-marked PVCs to form typical enlarged structures. By contrast, wortmannin caused YFP-labeled PVCs but not YFP-labeled Golgi stacks to vacuolate. VSR antibodies labeled multivesicular bodies (MVBs) on thin sections prepared from high-pressure frozen/freeze substituted samples, and the enlarged PVCs also were indentified as MVBs. MVBs were further purified from BY-2 cells and found to contain VSR proteins via immunogold negative staining. Similar to YFP-labeled Golgi stacks, YFP-labeled PVCs are mobile organelles in BY-2 cells. Thus, we have unequivocally identified MVBs as PVCs in N. tabacum BY-2 cells. Uptake studies with the styryl dye FM4-64 strongly indicate that PVCs also lie on the endocytic pathway of BY-2 cells.
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