ArticleJournal of bacteriology2004
Identification of a 349-kilodalton protein (Gli349) responsible for cytadherence and glass binding during gliding of Mycoplasma mobile.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 102 citations in OpenAlex.
- Gliding direction ofBiophysics and physicobiology · 2025Article
- Rapid in vitro method to assemble and transfer DNA fragments into the JCVI-syn3B minimal synthetic bacterial genome through Cre/Biophysics and physicobiology · 2024Article
- Internal structure ofBiophysics and physicobiology · 2024Article
- Structure and Function of Gli123 Involved in Mycoplasma mobile Gliding.Journal of bacteriology · 2023Article
- Detection of Steps and Rotation in the Gliding Motility of Mycoplasma mobile.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Purification and Structural Analysis of the Gliding Motility Machinery in Mycoplasma mobile.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Isolation and structure of the fibril protein, a major component of the internal ribbon forFrontiers in microbiology · 2022Article
- Chained Structure of Dimeric FmBio · 2021Article
- Movements of Mycoplasma mobile Gliding Machinery Detected by High-Speed Atomic Force Microscopy.mBio · 2021Article
- Article
- Force and Stepwise Movements of Gliding Motility in Human Pathogenic BacteriumFrontiers in microbiology · 2021Article
- Article
- Cytophaga hutchinsoniiApplied and environmental microbiology · 2020Article
- Identification and sequence analyses of the gliding machinery proteins from Mycoplasma mobile.Scientific reports · 2020Article
- Article
- Behaviors and Energy Source ofJournal of bacteriology · 2019Article
- Linear motor driven-rotary motion of a membrane-permeabilized ghost in Mycoplasma mobile.Scientific reports · 2018Article
- Detailed Analyses of Stall Force Generation in Mycoplasma mobile Gliding.Biophysical journal · 2018Article
- Novel mechanisms power bacterial gliding motility.Molecular microbiology · 2016Review
- Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several mycoplasma species are known to glide in the direction of the membrane protrusion (head-like structure), but the mechanism underlying this movement is entirely unknown. To identify proteins involved in the gliding mechanism, protein fractions of Mycoplasma mobile were analyzed for 10 gliding mutants isolated previously. One large protein (Gli349) was observed to be missing in a mutant m13 deficient in hemadsorption and glass binding. The predicted amino acid sequence indicated a 348,758-Da protein that was truncated at amino acid residue 1257 in the mutant. Immunofluorescence microscopy with a monoclonal antibody showed that Gli349 is localized at the head-like protrusion's base, which we designated the cell neck, and immunoelectron microscopy established that the Gli349 molecules are distributed all around this neck. The number of Gli349 molecules on a cell was estimated by immunoblot analysis to be 450 +/- 200. The antibody inhibited both the hemadsorption and glass binding of M. mobile. When the antibody was used to treat gliding mycoplasmas, the gliding speed and the extent of glass binding were inhibited to similar extents depending on the concentration of the antibody. This suggested that the Gli349 molecule is involved not only in glass binding for gliding but also in movement. To explain the present results, a model for the mechanical cycle of gliding is discussed.
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