ArticleTransactions of the American Ophthalmological Society2003
Analysis of retinal pigment epithelium integrin expression and adhesion to aged submacular human Bruch's membrane.
Article in Transactions of the American Ophthalmological Society, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- Desmosome and Hemidesmosome Release via Exosomes from Retinal Pigmented Epithelium - A Precursor to Epithelial-Mesenchymal Transition in Early AMD?Current eye research · 2025Review
- Bruch's-Mimetic Nanofibrous Membranes Functionalized with the Integrin-Binding Peptides as a Promising Approach for Human Retinal Pigment Epithelium Cell Transplantation.Molecules (Basel, Switzerland) · 2022Article
- AMD-Like Substrate Causes Epithelial Mesenchymal Transition in iPSC-Derived Retinal Pigment Epithelial Cells Wild Type but NotInternational journal of molecular sciences · 2021Article
- FHL-1 interacts with human RPE cells through the α5β1 integrin and confers protection against oxidative stress.Scientific reports · 2021Article
- Human Stem Cell-Derived Retinal Pigment Epithelial Cells as a Model for Drug Screening and Pre-Clinical Assays Compared to ARPE-19 Cell Line.International journal of stem cells · 2021Article
- Culture surface protein coatings affect the barrier properties and calcium signalling of hESC-RPE.Scientific reports · 2021Article
- Anti-integrin therapy for retinovascular diseases.Expert opinion on investigational drugs · 2020Review
- Risuteganib Protects against Hydroquinone-induced Injury in Human RPE Cells.Investigative ophthalmology & visual science · 2020Article
- Induction of posterior vitreous detachment (PVD) by non-enzymatic reagents targeting vitreous collagen liquefaction as well as vitreoretinal adhesion.Scientific reports · 2020Article
- Stem cell-derived retinal pigment epithelium from patients with age-related macular degeneration exhibit reduced metabolism and matrix interactions.Stem cells translational medicine · 2020Article
- Recent Advances in Retinal Stem Cell Therapy.Current molecular biology reports · 2017Article
- Angiopoietin-like Protein 2 Is a Multistep Regulator of Inflammatory Neovascularization in a Murine Model of Age-related Macular Degeneration.The Journal of biological chemistry · 2016Article
- Two Bioactive Molecular Weight Fractions of a Conditioned Medium Enhance RPE Cell Survival on Age-Related Macular Degeneration and Aged Bruch's Membrane.Translational vision science & technology · 2016Article
- Enhancing RPE Cell-Based Therapy Outcomes for AMD: The Role of Bruch's Membrane.Translational vision science & technology · 2014Article
- Structure and barrier properties of human embryonic stem cell-derived retinal pigment epithelial cells are affected by extracellular matrix protein coating.Tissue engineering. Part A · 2014Article
- Montmorillonite clay based polyurethane nanocomposite as substrate for retinal pigment epithelial cell growth.Journal of materials science. Materials in medicine · 2013Article
- Comparison of FRPE and human embryonic stem cell-derived RPE behavior on aged human Bruch's membrane.Investigative ophthalmology & visual science · 2011Article
- Cell-deposited matrix improves retinal pigment epithelium survival on aged submacular human Bruch's membrane.Investigative ophthalmology & visual science · 2011Article
- Integrin alpha5beta1 mediates attachment, migration, and proliferation in human retinal pigment epithelium: relevance for proliferative retinal disease.Investigative ophthalmology & visual science · 2009Article
- Progressive morphological and functional defects in retinas from alpha1 integrin-null mice.Investigative ophthalmology & visual science · 2008Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeUncultured aged retinal pigment epithelium (RPE) does not resurface aged Bruch's membrane after 24 hours in organ culture. These experiments assess whether culturing alters RPE integrin expression and resurfacing of Bruch's membrane.
methodsRNA was isolated from uncultured and cultured RPE of aged adult donor and fetal eyes. Integrin subunit messenger RNA (mRNA) expression was studied by reverse transcriptase-polymerase chain reaction (RT-PCR) and semiquantitative analysis of the amplified products. Cell surface integrin expression was assessed using flow cytometry. Passaged cultured fetal RPE and primary cultured aged RPE were seeded onto Bruch's membrane, and resurfacing was assessed with scanning electron microscopy.
resultsUncultured fetal RPE had low levels of alpha3 and beta5 mRNA compared to passaged cultured fetal RPE. Uncultured aged RPE had decreased alpha1-5 mRNA compared to primary cultured aged RPE. Cultured aged RPE had decreased beta4 and beta5 mRNA compared to passaged cultured fetal RPE. Flow cytometry confirmed the expression of alpha1-5, alphav, and beta1 protein on cultured fetal RPE and alpha1-3 and beta1 protein on cultured aged RPE. Twenty-four hours after seeding, cultured fetal and aged RPE resurfaced 99% +/- 1.3% and 76% +/- 22%, respectively, of aged submacular Bruch's membrane specimens from which native RPE had been debrided, exposing the native RPE basement membrane. Cultured fetal and aged RPE resurfaced 97% +/- 3.1% and 39% +/- 35%, respectively, of specimens in which the inner collagenous layer was exposed.
conclusionsUncultured aged RPE has low amounts of integrin subunits that form receptors for laminin, fibronectin, and collagens. Culturing up-regulates integrins and promotes more efficient aged RPE attachment to and survival on aged Bruch's membrane.
Indexed as
Identifiers
14971591PMC1359002W2128421857What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.