ArticleRNA (New York, N.Y.)2004
Characterization of deadenylation in trypanosome extracts and its inhibition by poly(A)-binding protein Pab1p.
Article in RNA (New York, N.Y.), 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Uncovering the therapeutic potential of anti-tuberculoid agent Isoniazid in a model of microbial-driven Crohn's disease.Journal of Crohn's & colitis · 2025Article
- The Role of KH-Type Splicing Regulatory Protein (KSRP) for Immune Functions and Tumorigenesis.Cells · 2022Review
- The roles of 3'-exoribonucleases and the exosome in trypanosome mRNA degradation.RNA (New York, N.Y.) · 2013Article
- The protein Zfand5 binds and stabilizes mRNAs with AU-rich elements in their 3'-untranslated regions.The Journal of biological chemistry · 2012Article
- Inhibition of mRNA maturation in trypanosomes causes the formation of novel foci at the nuclear periphery containing cytoplasmic regulators of mRNA fate.Journal of cell science · 2012Article
- The role of the 5'-3' exoribonuclease XRNA in transcriptome-wide mRNA degradation.RNA (New York, N.Y.) · 2011Article
- PolyA-specific ribonuclease (PARN-1) function in stage-specific mRNA turnover in Trypanosoma brucei.Eukaryotic cell · 2011Article
- RNA Granules Living a Post-transcriptional Life: the Trypanosomes' Case.Current chemical biology · 2011Article
- Functional characterization of three leishmania poly(a) binding protein homologues with distinct binding properties to RNA and protein partners.Eukaryotic cell · 2010Article
- Identification of the HIT-45 protein from Trypanosoma brucei as an FHIT protein/dinucleoside triphosphatase: substrate specificity studies on the recombinant and endogenous proteins.RNA (New York, N.Y.) · 2009Article
- Functionally related transcripts have common RNA motifs for specific RNA-binding proteins in trypanosomes.BMC molecular biology · 2008Article
- Inhibition of tristetraprolin deadenylation by poly(A) binding protein.American journal of physiology. Gastrointestinal and liver physiology · 2008Article
- A role for Caf1 in mRNA deadenylation and decay in trypanosomes and human cells.Nucleic acids research · 2008Article
- The 3' untranslated region of sindbis virus represses deadenylation of viral transcripts in mosquito and Mammalian cells.Journal of virology · 2008Article
- RNA-binding domain proteins in Kinetoplastids: a comparative analysis.Eukaryotic cell · 2005Article
- Opposing effects of polyadenylation on the stability of edited and unedited mitochondrial RNAs in Trypanosoma brucei.Molecular and cellular biology · 2005Article
- Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.RNA (New York, N.Y.) · 2004Article
- Kiss your tail goodbye: the role of PARN, Nocturnin, and Angel deadenylases in mRNA biology.Biochimica et biophysica actaReview
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The stability of mRNAs is an important point in the regulation of gene expression in eukaryotes. The mRNA turnover pathways have been identified in yeast and mammals. However, mRNA turnover pathways in trypanosomes have not been widely studied. Deadenylation is the first step in the major mRNA turnover pathways of yeast and mammals. To better understand mRNA degradation processes in these organisms, we have developed an in vitro mRNA turnover system that is functional for deadenylation. In this system, addition of poly(A) homopolymer activates the deadenylation of poly(A) tails. The trypanosomal deadenylase activity is a 3'-->5' exonuclease specific for adenylate residues, generates 5'-AMP as a product, is magnesium dependent, and is inhibited by neomycin B sulfate. These characteristics suggest similarity with other eukaryotic deadenylases. Furthermore, this activity is cap independent, indicating a potential difference between the trypanosomal activity and PARN, but suggesting similarity to Ccr4p/Pop2p activities. Extracts immunodepleted of Pab1p required the addition of poly(A) competition to activate deadenylation. Trypanosomal Pab1p functions as an inhibitor of the activity under in vitro conditions. Pab1p appears to be one of several mRNA stability proteins in trypanosomal extracts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.