ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
Positional cloning of the rice Rf-1 gene, a restorer of BT-type cytoplasmic male sterility that encodes a mitochondria-targeting PPR protein.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 251 citations in OpenAlex.
- Non-functional allele ofPlant biotechnology (Tokyo, Japan) · 2026Article
- A PPR gene cluster from Solanum aethiopicum underlies fertility restoration in cytoplasmic male-sterile eggplant (S. melongena L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- From High-Density Genomic Mapping to Precision Molecular Breeding: A Comprehensive Review ofGenes · 2026Review
- Genetic mapping of fertility restoration (Rf) genes and development-validation of linked SNPs for AJournal of applied genetics · 2026Article
- Energy Metabolism and Auxin Signaling Disruption Underlying Stamen Identity Defects in Tobacco Cytoplasmic Male Sterility K326 (CMS K326): Integrated Transcriptomic and Metabolomic Analyses.Plants (Basel, Switzerland) · 2026Article
- Advancements in hybrid rice production: improvements in male sterility and synthetic apomixis for sustainable agriculture.Plant biotechnology journal · 2025Review
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- Identification of quantitative trait loci for yield traits and fine-mapping ofFrontiers in plant science · 2025Article
- Reference-based genome assembly and comparative genomics of Calamus Brandisii Becc. for unveiling sex-specific genes for early gender detection.Functional & integrative genomics · 2024Article
- Natural variation in the promoter of qRBG1/OsBZR5 underlies enhanced rice yield.Nature communications · 2024Article
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- Development of Single-Segment Substitution Lines and Fine-Mapping ofInternational journal of molecular sciences · 2023Article
- Fine mapping and candidate gene analysis of a novel fertility restorer gene for C-type cytoplasmic male sterility in maize (Zea mays L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Genomic Resequencing Unravels the Genetic Basis of Domestication, Expansion, and Trait Improvement in Morus Atropurpurea.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Genomic survey of high-throughput RNA-Seq data implicates involvement of long intergenic non-coding RNAs (lincRNAs) in cytoplasmic male-sterility and fertility restoration in pigeon pea.Genes & genomics · 2023Article
- The Alpha Subunit of Mitochondrial Processing Peptidase Participated in Fertility Restoration in Honglian-CMS Rice.International journal of molecular sciences · 2023Article
- Induction of Male Sterility by Targeted Mutation of a Restorer-of-Fertility Gene with CRISPR/Cas9-Mediated Genome Editing inPlants (Basel, Switzerland) · 2022Article
- QTL Analysis of Z414, a Chromosome Segment Substitution Line with Short, Wide Grains, and Substitution Mapping of qGL11 in Rice.Rice (New York, N.Y.) · 2022Article
- Identification, pyramid, and candidate gene of QTL for yield-related traits based on rice CSSLs inMolecular breeding : new strategies in plant improvement · 2022Article
46 more citing papers are in PubMed but not listed here.
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7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of cytoplasmic male sterility (CMS) in one parent and a restorer gene ( Rf) to restore fertility in another are indispensable for the development of hybrid varieties. We have found a rice Rf-1 gene that restores BT-type CMS by applying a positional cloning strategy. Using linkage analysis in combination with 6,104 BC(1)F(3) progeny derived from a cross between two near-isogenic lines (NILs) differing only at the Rf-1 locus, we delimited the Rf-1 gene to a 22.4-kb region in the rice genome. Duplicate open reading frames ( Rf-1A and Rf-1B) with a pentatricopeptide (PPR) motif were found in this region. Since several insertions and/or deletions were found in the regions corresponding to both the Rf-1A and Rf-1B genes in the maintainer's allele, they may have lost their function. Rf-1A protein had a mitochondria-targeting signal, whereas Rf-1B did not. The Rf-1B gene encoded a shorter polypeptide that was determined by a premature stop codon. Based on the function of the Rf-1 gene, its product is expected to target mitochondria and may process the transcript from an atp6/orf79 region in the mitochondrial genome. Since the Rf-1A gene encodes a 791-amino acid protein with a signal targeting mitochondria and has 16 repeats of the PPR motif, we concluded that Rf-1A is the Rf-1 gene. Nine duplications of Rf-1A homologs were found around the Rf-1 locus in the Nipponbare genome. However, while some of them encoded proteins with the PPR motif, they do not restore BT-type CMS based on the lack of co-segregation with the restoration phenotype. These duplicates may have played diversified roles in RNA processing and/or recombination in mitochondria during the co-evolution of these genes and the mitochondrial genome.
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