ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2004
High-oleate peanut mutants result from a MITE insertion into the FAD2 gene.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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38 citing papers in PubMed, 161 citations in OpenAlex.
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- Miniature Inverted-Repeat Transposable Elements: Small DNA Transposons That Have Contributed to PlantPlants (Basel, Switzerland) · 2023Review
- Understanding variation in oleic acid content of high-oleic virginia-type peanut.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Comparative repeatome analysis reveals new evidence on genome evolution in wild diploid Arachis (Fabaceae) species.Planta · 2022Article
- Identification and characterization of transposable element AhMITE1 in the genomes of cultivated and two wild peanuts.BMC genomics · 2022Article
- Breeding of a new variety of peanut with high-oleic-acid content and high-yield by marker-assisted backcrossing.Molecular breeding : new strategies in plant improvement · 2022Article
- Genome-wide approaches delineate the additive, epistatic, and pleiotropic nature of variants controlling fatty acid composition in peanut (Arachis hypogaea L.).G3 (Bethesda, Md.) · 2022Article
- Towards a Cardoon (International journal of molecular sciences · 2021Article
- Tailoring the Structure of Lipids, Oleogels and Fat Replacers by Different Approaches for Solving theFoods (Basel, Switzerland) · 2021Review
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- Miniature inverted-repeat transposable elements (MITEs), derived insertional polymorphism as a tool of marker systems for molecular plant breeding.Molecular biology reports · 2020Review
- Combining High Oleic Acid Trait and Resistance to Late Leaf Spot and Rust Diseases in Groundnut (Frontiers in genetics · 2020Article
- Transcriptomic Analysis Reveals the High-Oleic Acid Feedback Regulating the Homologous Gene Expression of Stearoyl-ACP Desaturase 2 (International journal of molecular sciences · 2019Article
- Marker-assisted backcrossing to improve seed oleic acid content in four elite and popular peanut (Breeding science · 2019Article
- Heterochromatin evolution in Arachis investigated through genome-wide analysis of repetitive DNA.Planta · 2019Article
- Mutagenesis of FAD2 genes in peanut with CRISPR/Cas9 based gene editing.BMC biotechnology · 2019Article
- Article
- The Evolution and Biocatalysis of FAD2 Indicate Its Correlation to the Content of Seed Oil in Plants.International journal of molecular sciences · 2019Article
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A high-oleate trait in the cultivated peanut ( Arachis hypogaea L.) was reported to rely on the allelic composition of the two homeologous, microsomal oleoyl-PC desaturase genes ( ahFAD2A or ahFAD2B). The enzyme activity of either ahFAD2A or ahFAD2B is sufficient for a normal oleate phenotype, and a significant reduction in the levels of ahFAD2B and a mutation in ahFAD2A were reported to be responsible for the high-oleate phenotype in one chemically induced mutant (M2-225) and one derived from a naturally occurring (8-2122) mutant. Here, we report an insertion of the same miniature inverted-repeat transposable element (MITE) in the ahFAD2B gene in another chemically induced mutant (Mycogen-Flavo) and the previously characterized M2-225 mutant. In both cases, this MITE insertion in ahFAD2B causes a frameshift, resulting in a putatively truncated protein sequence in both mutants. The insertion of this MITE in ahFAD2B, in addition to the point mutation in ahFAD2A, appears to be the cause of the high-oleate phenotype in Mycogen-Flavo and M2-225 mutants. Utilizing sequences of the MITE, we developed a DNA marker strategy to differentiate the two insertion-containing mutants from the normal oleate peanut variety (AT-108) and the naturally occurring, high-oleate mutant 8-2122. Reverse transcript-PCR/differential digestion results reveal the expression of both ahFAD2A and ahFAD2B genes in Mycogen-Flavo mutant. This result is in contrast to the observation that ahFAD2B transcripts are greatly reduced in the M2-225 mutant having the MITE insertion further 3' in ahFAD2B gene.
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