ArticleWorld journal of gastroenterology2004
Insulin expression in livers of diabetic mice mediated by hydrodynamics-based administration.
Article in World journal of gastroenterology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
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- Preclinical and clinical advances in transposon-based gene therapy.Bioscience reports · 2017Review
- Role of recombinant plasmid pEGFP-N1-IGF-1 transfection in alleviating osteoporosis in ovariectomized rats.Journal of molecular histology · 2013Article
- Retargeting transposon insertions by the adeno-associated virus Rep protein.Nucleic acids research · 2012Article
- Hydrodynamic gene delivery and its applications in pharmaceutical research.Pharmaceutical research · 2011Review
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- A transposon and transposase system for human application.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Review
- In vivo gene delivery by cationic tetraamino fullerene.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Gene expression in lung and liver after intravenous infusion of polyethylenimine complexes of Sleeping Beauty transposons.Human gene therapy · 2010Article
- In vivo inhibition of focal adhesion kinase causes insulin resistance.The Journal of physiology · 2008Article
- Pigs taking wing with transposons and recombinases.Genome biology · 2007Review
- Variability of naked DNA expression after direct local injection: the influence of the injection speed.Gene therapy · 2006Article
- Role of protein tyrosine phosphatases in cancer.Progress in nucleic acid research and molecular biology · 2006Review
- High-resolution genome-wide mapping of transposon integration in mammals.Molecular and cellular biology · 2005Article
- Nonviral in vivo gene transfer in the mucopolysaccharidosis I murine model.Journal of inherited metabolic disease · 2005Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTransfer and expression of insulin gene in vivo are an alternative strategy to improve glycemic control in type 1 diabetes. Hydrodynamics-based procedure has been proved to be very efficient to transfer naked DNA to mouse livers. The basal hepatic insulin production mediated by this rapid tail vein injection was studied to determine its effect on the resumption of glycemic control in type 1 diabetic mice.
methodsEngineered insulin cDNA was inserted into plasmid vectors under a CMV promoter, and transferred into STZ induced diabetic mice by hydrodynamic procedure. Glucose levels, body weight of treated mice, insulin levels, immunohistology of the liver, and quantity of insulin mRNA in the liver were assayed to identify the improvement of hyperglycemic complication after plasmid administration. Sleeping Beauty, a transposon system, was also used to prolong the insulin expression in the liver.
resultsAfter plasmid administration, Plasma insulin was significantly increased in the diabetic mice and the livers were insulin-positive by immunostaining. At the same time the hyperglycemic complication was improved. The blood glucose levels of mice were reduced to normal. Glucose tolerance of the treated diabetic mice was improved. Body weight loss was also ameliorated. The rapid tail vein injection did not cause any fatal result.
conclusionOur results suggested that insulin gene could be efficiently transferred into the livers of diabetic mice via rapid tail vein injection and it resulted in high level of insulin expression. The basal hepatic insulin production mediated by hydrodynamics-based administration improved the glycemic control in type 1 diabetes dramatically and ameliorated diabetic syndromes. Hydrodynamics-based administration offers a simple and efficient way in the study of gene therapy for type 1 diabetes.
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